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Western blot - U1A antibody (ab40689)
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Immunocytochemistry/ Immunofluorescence - U1A antibody (ab40689)
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Product Name
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U1A antibody
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See all U1A antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to U1A
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human U1A. (Peptide available as ab41553.)
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Reacts with
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Hu, Dm
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Predicted to react
(species key)
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Ms, Cow
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK293 (Human embryonic kidney cell line)
MCF7 (Human breast adenocarcinoma cell line)
SHSY-5Y (Human neuroblastoma cell line)
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
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Relevance
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U1A binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) it may be involved in coupled pre-mRNA splicing and polyadenylation process.
It belongs to the spliceosome where it is associated with snRNP U1. It interacts with SFPQ (referenced from swissprot).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.30 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this U1A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for U1A antibody images, references, products related to ab40689 and other tools.
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U1A antibody images:
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 Western blot - U1A antibody (ab40689)
All lanes : U1A antibody (ab40689) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The U1A protein is acetylated and rich in proline residues (SwissProt data) which may cause it to run at a higher molecular weight than predicted.
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Immunocytochemistry/ Immunofluorescence - U1A antibody (ab40689)
ICC/IF image of ab40689 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40689, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to U1A
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U1A antibody - more information
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Customer reviews (feedback) regarding U1A antibody |
Customer FAQs regarding U1A antibody |
Protocols for U1A antibody |
Price and availability of products related to U1A antibody |
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U1A antibody - related products:
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U1A antibody - other tools:
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Contact Abcam with a Technical Enquiry about U1A antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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