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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Overview
Product name
Anti-U1A antibody
See all U1A products (3) ...
Description
Rabbit polyclonal to U1A
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human, Fruit fly (Drosophila melanogaster)
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human U1A.
(Peptide available as ab41553.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
Sequence similarities
Belongs to the RRM U1 A/B'' family.
Contains 2 RRM (RNA recognition motif) domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09012
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Mud1 antibody
- small nuclear ribonucleoprotein polypeptide A antibody
- snRNP A antibody
see all
Database links
- Entrez Gene: 31442 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 6626 Human
- Entrez Gene: 53607 Mouse
- Omim: 182285 Human
- SwissProt: P43332 Fruit fly (Drosophila melanogaster)
- SwissProt: P09012 Human
- SwissProt: Q62189 Mouse
- SwissProt: Q9CXX7 Mouse
see all
Anti-U1A antibody images:
Western blot - U1A antibody (ab40689)
All lanes : Anti-U1A antibody (ab40689) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The U1A protein is acetylated and rich in proline residues (SwissProt data) which may cause it to run at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - U1A antibody (ab40689)
ICC/IF image of ab40689 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40689, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-U1A antibody (ab40689)
Anti-U1A antibody (ab40689) at 1/200 dilution + NSC34 cell nuclear extract at 20 µg
Secondary
IRDye® 800CW Donkey anti-Rabbit IgG at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
Exposure time : 1 minute
This image is courtesy of an Anonymous Abreview.
References for Anti-U1A antibody (ab40689)
This product has been referenced in:
- Brody Yet al. The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing. PLoS Biol 9:e1000573 (2011). ICC/IF; Human.Read more (PubMed: 21264352) »
- Bandhakavi Set al. Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response. Mol Cell Proteomics 9:403-14 (2010). WB; Human.Read more (PubMed: 19955088) »
See all 2 publications for this product
Publishing research using ab40689? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"