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IHC-P - U1A antibody (ab55751)
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Western blot - U1A antibody (ab55751)
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Immunocytochemistry/ Immunofluorescence-U1A antibody(ab55751)
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Product Name
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U1A antibody
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See all U1A antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to U1A
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Immunogen
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Recombinant full length protein, corresponding to amino acids 1-283 of Human U1A
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
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Relevance
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U1A binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) it may be involved in coupled pre-mRNA splicing and polyadenylation process.
It belongs to the spliceosome where it is associated with snRNP U1. It interacts with SFPQ (referenced from swissprot).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this U1A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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U1A antibody images:
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 IHC-P - U1A antibody (ab55751)
U1A antibody (ab55751) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart tissue.
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Western blot - U1A antibody (ab55751)
Predicted band size : 31 kDa
U1A antibody (ab55751) at 1ug/lane + HeLa cell lysate at 25ug/lane.
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Immunocytochemistry/ Immunofluorescence-U1A antibody(ab55751)
ab55751 at 10 ug/ml staining U1A in human Hella cells by Immunocytochemistry/ Immunofluorescence.
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Search PubMed (MEDLINE) for references to U1A
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U1A antibody - more information
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Customer reviews (feedback) regarding U1A antibody |
Customer FAQs regarding U1A antibody |
Protocols for U1A antibody |
Price and availability of products related to U1A antibody |
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U1A antibody - other tools:
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Contact Abcam with a Technical Enquiry about U1A antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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