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All lanes : Anti-U2AF1L3 antibody (ab73033) at 1/500 dilution
Lane 1 : U2AF1L3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 22 kDa
Observed band size : 22 kDa
Note: Predicted molecular weight for the isoform 1 full length protein is 25 kDa, whereas that for isoform 2 (the immunogen) is 22 kDa.
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