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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Overview
Product name
Anti-U2AF1L3 antibody
See all U2AF1L3 products (3) ...
Description
Mouse polyclonal to U2AF1L3
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length human U2AF1L3 protein, isoform 2 (Q8WU68-2)
Positive control
U2AF1L3 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - U2AF1L3 antibody (ab73033)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
- 1 Image
WB
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
Note: this antibody requires purification for this application
WB: 1/500 - 1/1000. Predicted molecular weight: isoform 1, 25 kDa; isoform 2, 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site.
Tissue specificity
Isoform 2 is widely expressed. Isoform 3 is highly expressed in heart, brain and lung, lower expressed in thymus and much lower expressed in peripheral blood leukocytes.
Sequence similarities
Belongs to the splicing factor SR family.
Contains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain.
Domain
The second zinc finger in necessary for interaction with GFI1 and for alternative pre-mRNA splicing events.
Cellular localization
Nucleus. Nucleus speckle.
Target information above from: UniProt accessionQ8WU68
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ35525 antibody
- MGC33901 antibody
- Splicing factor U2AF 26 kDa subunit antibody
see all
Database links
- Entrez Gene: 199746 Human
- Omim: 601080 Human
- SwissProt: Q8WU68 Human
- Unigene: 351558 Human
Anti-U2AF1L3 antibody images:
Western blot - U2AF1L3 antibody (ab73033)
All lanes : Anti-U2AF1L3 antibody (ab73033) at 1/500 dilution
Lane 1 : U2AF1L3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 22 kDa
Observed band size : 22 kDa
Note: Predicted molecular weight for the isoform 1 full length protein is 25 kDa, whereas that for isoform 2 (the immunogen) is 22 kDa.
References for Anti-U2AF1L3 antibody (ab73033)
ab73033 has not yet been referenced specifically in any publications.
Publishing research using ab73033? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"