You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-U2AF1L3 antibody (ab80607)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-U2AF1L3 antibody
See all U2AF1L3 products (3) ...

Description

Goat polyclonal to U2AF1L3

Specificity

ab80607 is expected to recognise isoforms 1 and 2 (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Dog

Immunogen

Synthetic peptide: C-EDGERAVAELSNR, corresponding to internal sequence amino acids 121 - 133 of Human U2AF1L3 (NP_001035515.1 and NP_659424.2)

C-EDGERAVA ELSNR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human Cerebellum lysate and lung lysates

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab80607 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing

  • Western blot - U2AF1L3 antibody (ab80607)Western blot - U2AF1L3 antibody (ab80607) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab80607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Peptide ELISA: antibody detection limit dilution 1/32000
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 26 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site.

Tissue specificity

Isoform 2 is widely expressed. Isoform 3 is highly expressed in heart, brain and lung, lower expressed in thymus and much lower expressed in peripheral blood leukocytes.

Sequence similarities

Belongs to the splicing factor SR family.
Contains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain.

Domain

The second zinc finger in necessary for interaction with GFI1 and for alternative pre-mRNA splicing events.

Cellular localization

Nucleus. Nucleus speckle.

Target information above from: UniProt accessionQ8WU68 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • FLJ35525 antibody
  • MGC33901 antibody
  • Splicing factor U2AF 26 kDa subunit antibody
  • U2 auxiliary factor 26 antibody
  • U2 small nuclear RNA auxiliary factor 1 like 3 antibody
  • U2 small nuclear RNA auxiliary factor 1 like 4 antibody
  • U2 small nuclear RNA auxiliary factor 1 like protein 3 antibody
  • U2 small nuclear RNA auxiliary factor 1 like protein 4 antibody
  • U2 small nuclear RNA auxiliary factor 1-like protein 3 antibody
  • U2 small nuclear RNA auxiliary factor 1-like protein 4 antibody
  • U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3 antibody
  • U2AF1 like protein 3 antibody
  • U2AF1 RS3 antibody
  • U2AF1-like protein 3 antibody
  • U2AF1L3V1 antibody
  • U2AF1L4 antibody
  • U2AF1RS3 antibody
  • U2af26 antibody
  • U2AF4_HUMAN antibody
  • U2RNU2 small nuclear RNA auxiliary factor 1 like 3 antibody
  • U2RNU2 small nuclear RNA auxiliary factor 1 like protein 3 antibody
see all

Database links

see all

Anti-U2AF1L3 antibody images:

  Western blot - U2AF1L3 antibody (ab80607)

Western blot - U2AF1L3 antibody (ab80607)

Anti-U2AF1L3 antibody (ab80607) at 0.1 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg

Predicted band size : 26 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)

References for Anti-U2AF1L3 antibody (ab80607)

ab80607 has not yet been referenced specifically in any publications.

Publishing research using ab80607? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"