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U937 (Human leukemic monocyte lymphoma cell line) Cytoplasmic Lysate - TPA stimulated (ab14905)

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Overview

  • Product nameU937 (Human leukemic monocyte lymphoma cell line) Cytoplasmic Lysate - TPA stimulatedSee all U937 lysates ...
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundThe U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

    • Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for U937 (Human leukemic monocyte lymphoma cell line) Cytoplasmic Lysate - TPA stimulated (ab14905)

    ab14905 has not yet been referenced specifically in any publications.

    Publishing research using ab14905 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"