U937 (Human leukemic monocyte lymphoma cell line) Cytoplasmic Lysate (ab14904)

Overview

  • Product nameU937 (Human leukemic monocyte lymphoma cell line) Cytoplasmic LysateSee all U937 lysates ...
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundThe U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for U937 (Human leukemic monocyte lymphoma cell line) Cytoplasmic Lysate (ab14904)

    ab14904 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. U937 cells grow in suspension. To make them adhere, you can try using poly-lysine, fibronectin or collagen coated plates. I hope this information helps, please do not hesitate to contact us if you need any more advice...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"