Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Overview
Product name
U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
See all U937 products (6) ...
General notes
Cell line: U937 (Human histiocytic lymphoma)
Growth media: DMEM & 10% NCS (Newborn Calf serum).
Tested applications
Properties
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Lysate notes
U937 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Background
The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (Ready to load; 10 - 20 µg/lane is recommended for mini gel.)
References for U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate (ab3959)
This product has been referenced in:
- Lau JYet al. Differential expression of peroxisome proliferator activated receptor gamma and cyclin D1 does not affect proliferation of asthma- and non-asthma-derived airway smooth muscle cells. Respirology 15:303-12 (2010). WB.Read more (PubMed: 20070588) »
See 1 publication for this product
Publishing research using ab3959? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"