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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab84478? Please let us know so that we can cite the reference in this datasheet
ab84478 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UACA antibody (ab84478)
ab84478, at a 1/250 dilution, staining UACA in formalin fixed, paraffin embedded human ovarian carcinoma tissue section by Immunohistochemistry. Detection: DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UACA antibody (ab84478)
ab84478, at a 1/250 dilution, staining UACA in formalin fixed, paraffin embedded human breast carcinoma tissue section by Immunohistochemistry.
Detection: DAB staining
Immunocytochemistry/ Immunofluorescence - Anti-UACA antibody (ab84478)
ICC/IF image of ab84478 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84478, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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