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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-UACA antibody
See all UACA products (4) ...
Description
Rabbit polyclonal to UACA
Tested applications
ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to amino acids 675-725 (KAKLAQHVKP EEHEQVKSRL EQKSGELGKK ITELTLKNQT LQKEIEKVYL D) of human UACA (NP_060473.2).
KAKLAQHVKP EEHEQVKSRL EQKSGELGKK ITELTLKNQT LQKEIEKVYL D
Positive control
Human controls: Anaplastic thyroid carcinoma, breast carcinoma, colon carcinoma, Linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, stomach adenocarcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: 1/100 - 1/500.
Target
Relevance
UACA1 regulates APAF1 expression and plays an important role in the regulation of stress induced apoptosis. It promotes apoptosis by regulating three pathways, apoptosome up regulation, LGALS3/galectin 3 down regulation and NF kappa B inactivation. It regulates the redistribution of APAF1 into the nucleus after proapoptotic stress and down regulates the expression of LGALS3 by inhibiting NFKB1. UACA modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.
Cellular localization
Nucleus. Cytoplasm, cytoskeleton. Note: Expressed diffusely in cytoplasm.
Alternative names
- FLJ10128 antibody
- KIAA1561 antibody
- MGC141967 antibody
see all
Database links
- Entrez Gene: 55075 Human
- Omim: 612516 Human
- SwissProt: Q9BZF9 Human
Anti-UACA antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UACA antibody (ab84478)
ab84478, at a 1/250 dilution, staining UACA in formalin fixed, paraffin embedded human ovarian carcinoma tissue section by Immunohistochemistry. Detection: DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UACA antibody (ab84478)
ab84478, at a 1/250 dilution, staining UACA in formalin fixed, paraffin embedded human breast carcinoma tissue section by Immunohistochemistry.
Detection: DAB staining
Immunocytochemistry/ Immunofluorescence - Anti-UACA antibody (ab84478)
ICC/IF image of ab84478 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84478, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-UACA antibody (ab84478)
ab84478 has not yet been referenced specifically in any publications.
Publishing research using ab84478? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"