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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-UBA5 antibody - N-terminal
See all UBA5 products (4) ...
Description
Rabbit polyclonal to UBA5 - N-terminal
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N-terminal region of human UBA5 conjugated to KLH
Positive control
Mouse kidney tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab71422 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
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Western blot - UBA5 antibody - N-terminal (ab71422)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Target
Function
E1-like enzyme which activates UFM1 and SUMO2.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.
Cellular localization
Cytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2.
Target information above from: UniProt accessionQ9GZZ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5730525G14Rik antibody
- AW240750 antibody
- FLJ23251 antibody
see all
Database links
- Entrez Gene: 79876 Human
- Omim: 610552 Human
- SwissProt: Q9GZZ9 Human
- Unigene: 170737 Human
Anti-UBA5 antibody - N-terminal images:
Western blot - UBA5 antibody - N-terminal (ab71422)
Anti-UBA5 antibody - N-terminal (ab71422) at 1/100 dilution + mouse kidney tissue lysate at 12.5 µg
Predicted band size : 45 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa,35 kDa,37 kDa,80 kDa. We are unsure as to the identity of these extra bands.
References for Anti-UBA5 antibody - N-terminal (ab71422)
ab71422 has not yet been referenced specifically in any publications.
Publishing research using ab71422? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"