Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-UBA5 antibody
See all UBA5 products (4) ...
Description
Mouse polyclonal to UBA5
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human) (NP_079094.1)
Positive control
Human colon tissue lysate; UBA5 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Target
Function
E1-like enzyme which activates UFM1 and SUMO2.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.
Cellular localization
Cytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2.
Target information above from: UniProt accessionQ9GZZ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5730525G14Rik antibody
- AW240750 antibody
- FLJ23251 antibody
see all
Database links
- Entrez Gene: 79876 Human
- Omim: 610552 Human
- SwissProt: Q9GZZ9 Human
- Unigene: 170737 Human
Anti-UBA5 antibody images:
Western blot - UBA5 antibody (ab69046)
Anti-UBA5 antibody (ab69046) at 1/500 dilution + Human colon tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 45 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
Western blot - UBA5 antibody (ab69046)
All lanes : Anti-UBA5 antibody (ab69046) at 1/500 dilution
Lane 1 : UBA5 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 45 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-UBA5 antibody (ab69046)
ab69046 has not yet been referenced specifically in any publications.
Publishing research using ab69046? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"