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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-UBC3B antibody
See all UBC3B products (3) ...
Description
Goat polyclonal to UBC3B
Tested applications
WB, ELISA, Electron Microscopymore details
Cross reactivity
Reacts with
Rat
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide: AQQQMTSSQKALM, corresponding to N terminal amino acids 2-14 of Human UBC3B.
AQQQMTSSQK ALM-C
General notes
Principal Names – UBE2R2; UBC3B; CDC34B; FLJ20419; MGC10481; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B.
Official Gene Symbol - UBE2R2.
GenBank Accession Number – NP_060281.
LocusLink ID - 54926 (human); 67615 (mouse).
Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; protein modification; ligase activity.
An Ubiquitin-conjugating enzyme.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 µg/ml.)
ELISA: Use at an assay dependent concentration. (antibody detection limit dilution 1/4000.)
EM: Use at an assay dependent concentration. (This product stained chromatoid bodies in Rat spermatids.)
Target
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Target information above from: UniProt accessionQ712K3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDC34B antibody
- E2 CDC34B antibody
- FLJ20419 antibody
see all
Database links
- Entrez Gene: 54926 Human
- Entrez Gene: 67615 Mouse
- Entrez Gene: 299677 Rat
- Omim: 612506 Human
- SwissProt: Q712K3 Human
- SwissProt: Q6ZWZ2 Mouse
- Unigene: 725177 Human
- Unigene: 389540 Mouse
References for Anti-UBC3B antibody (ab4281)
This product has been referenced in:
- Haraguchi CMet al. Chromatoid bodies: aggresome-like characteristics and degradation sites for organelles of spermiogenic cells. J Histochem Cytochem 53:455-65 (2005).Read more (PubMed: 15805420) »
See 1 publication for this product
Publishing research using ab4281? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"