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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-UBE2J2 antibody
See all UBE2J2 products (2) ...
Description
Rabbit polyclonal to UBE2J2
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide: AVPNLAGLQQ ANRHHGLLGG ALANLFVIVG FAAFAYTVKY VLRSIAQE, corresponding to amino acids 211-259 of Human UBC6
AVPNLAGLQQ ANRHHGLLGG ALANLFVIVG FAAFAYTVKY VLRSIAQE
Positive control
HepG2 cell lysate; Human intestine tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay 1/312500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Cellular localization
Endoplasmic reticulum membrane.
Target information above from: UniProt accessionQ8N2K1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NCUBE-2 antibody
- NCUBE2 antibody
- Non-canonical ubiquitin-conjugating enzyme 2 antibody
see all
Database links
- Entrez Gene: 118424 Human
- Entrez Gene: 140499 Mouse
- SwissProt: Q2TA03 Cow
- SwissProt: Q5T7L1 Human
- SwissProt: Q8N2K1 Human
- SwissProt: Q6P073 Mouse
- Unigene: 191987 Human
- Unigene: 371673 Mouse
Anti-UBE2J2 antibody images:
Western blot - UBE2J2 antibody (ab59012)
Anti-UBE2J2 antibody (ab59012) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 29 kDa
Observed band size : 29 kDa
Diluted in 5% skim milk/PBS buffer; gel concentration 12%
Immunohistochemistry (Paraffin-embedded sections) - UBE2J2 antibody (ab59012)
Ab59012 at 4-8µg/ml staining epithelial cells of villus from human intestine; paraffin embedded; x400 magnification.
References for Anti-UBE2J2 antibody (ab59012)
ab59012 has not yet been referenced specifically in any publications.
Publishing research using ab59012? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"