Overview

  • Product nameAnti-UBC6e antibody
    See all UBC6e primary antibodies
  • Description
    Rabbit polyclonal to UBC6e
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human UBC6e.

    (Peptide available as ab39103.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

Properties

Applications

Our Abpromise guarantee covers the use of ab39104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human UBC6e peptide (ab39103).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionCatalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI-76 antibody
    • HSPC153 antibody
    • HSPC205 antibody
    • HSU93243 antibody
    • HSUBC6e antibody
    • MGC12555 antibody
    • NCUBE-1 antibody
    • NCUBE1 antibody
    • Non-canonical ubiquitin-conjugating enzyme 1 antibody
    • UB2J1_HUMAN antibody
    • UBC6 antibody
    • Ubc6p antibody
    • UBE2J1 antibody
    • Ubiquitin-conjugating enzyme E2 J1 antibody
    • Yeast ubiquitin-conjugating enzyme UBC6 homolog E antibody
    see all

Anti-UBC6e antibody images

  • All lanes : Anti-UBC6e antibody (ab39104) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
    Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab39104 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39104, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-UBC6e antibody (ab39104)

This product has been referenced in:
  • Bianchini E  et al. Unveiling the degradative route of the V247M a-sarcoglycan mutant responsible for LGMD-2D. Hum Mol Genet N/A:N/A (2014). WB ; Human . Read more (PubMed: 24565866) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"