Anti-UBC6e antibody (ab39104)

Overview

• Product nameAnti-UBC6e antibodySee all UBC6e primary antibodies ...
• Description
  Rabbit polyclonal to UBC6e
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human UBC6e.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab39103.)

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • Ubiquitination

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • Ubiquitin E2 Enzymes

Applications

Target

• FunctionCatalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
• Cellular localizationEndoplasmic reticulum membrane.

Target information above from: UniProt accession Q9Y385 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51465 Human
  • Entrez Gene: 51465 Human
  • SwissProt: Q9Y385 Human
  • SwissProt: Q9Y385 Human
  • Unigene: 163776 Human

• Alternative names
  CGI-76 antibodyHSPC153 antibodyHSPC205 antibody

  HSU93243 antibodyHSUBC6e antibodyMGC12555 antibodyNCUBE-1 antibodyNCUBE1 antibodyNon-canonical ubiquitin-conjugating enzyme 1 antibodyUB2J1_HUMAN antibodyUBC6 antibodyUbc6p antibodyUBE2J1 antibodyUbiquitin-conjugating enzyme E2 J1 antibodyYeast ubiquitin-conjugating enzyme UBC6 homolog E antibody

  see all

Anti-UBC6e antibody images

• 

  Western blot - UBC6e antibody (ab39104)
  All lanes : Anti-UBC6e antibody (ab39104) at 1/250 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
  Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
  Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
  Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
  Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 35 kDa
  Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - UBC6e antibody (ab39104)
  ICC/IF image of ab39104 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39104, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).