Overview

  • Product nameAnti-UBC6e antibodySee all UBC6e primary antibodies ...
  • Description
    Rabbit polyclonal to UBC6e
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cat, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: METRYNLKSP AVKRLMKEAA ELKDPTDHYH AQPLEDNLFE WHFTVRGPPD, corresponding to N terminal amino acids 1-50 of Human UBC6e

  • Positive control
    • Jurkat cell lysate, Human lung tissue, Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionCatalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI-76 antibody
    • HSPC153 antibody
    • HSPC205 antibody
    • HSU93243 antibody
    • HSUBC6e antibody
    • MGC12555 antibody
    • NCUBE-1 antibody
    • NCUBE1 antibody
    • Non-canonical ubiquitin-conjugating enzyme 1 antibody
    • UB2J1_HUMAN antibody
    • UBC6 antibody
    • Ubc6p antibody
    • UBE2J1 antibody
    • Ubiquitin-conjugating enzyme E2 J1 antibody
    • Yeast ubiquitin-conjugating enzyme UBC6 homolog E antibody
    see all

Anti-UBC6e antibody images

  • All lanes : Anti-UBC6e antibody (ab59014) at 2.5 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg/ml

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Diluted in 5% skim milk / PBS buffer; gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling UBC6e with ab59014 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling UBC6e with ab59014 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

References for Anti-UBC6e antibody (ab59014)

ab59014 has not yet been referenced specifically in any publications.

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