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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-UBCH6/UBE2E1 antibody
See all UBCH6/UBE2E1 products (2) ...
Description
Rabbit polyclonal to UBCH6/UBE2E1
Tested applications
IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human UBCH6/UBE2E1.
(Peptide available as ab38363.)
Positive control
This antibody gave a positive signal in HUVEC whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent concentration.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational
modifications
ISGylation suppresses ubiquitin E2 enzyme activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP51965
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- UB2E1_HUMAN antibody
- UbcH6 antibody
- UBE2E1 antibody
see all
Database links
- Entrez Gene: 7324 Human
- Omim: 602916 Human
- SwissProt: P51965 Human
- Unigene: 164853 Human
Anti-UBCH6/UBE2E1 antibody images:
Western blot - UBCH6/UBE2E1 antibody (ab36980)
Anti-UBCH6/UBE2E1 antibody (ab36980) at 1 µg/ml + HUVEC whole cell lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 21 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - UBCH6/UBE2E1 antibody (ab36980)
ICC/IF image of ab36980 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36980, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation - Anti-UBCH6/UBE2E1 antibody (ab36980)
UBCH6/UBE2E1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit polyclonal to UBCH6/UBE2E1 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36980. Secondary: Clean blot (HRP conjugate) at 1/1000 dilution. Bands: 25kDa: hnRNP A1; non specific - 18kDa: We are unsure as to the identity of this extra band.
References for Anti-UBCH6/UBE2E1 antibody (ab36980)
ab36980 has not yet been referenced specifically in any publications.
Publishing research using ab36980? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"