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Product Name
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UBCH6/UBE2E1 protein (His tag)
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Product type
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Proteins and Peptides
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Peptide
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Recombinant full length human UBCH6/UBE2E1 with His tag at N-terminal end, 21kDa, (SwissProt P51965).
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Tested applications
(see key)
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Conjugation, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
Conjugation: Use at an assay dependent dilution. Typical enzyme concentration to support conjugation in vitro is 100 nM-1 µM depending on conditions.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
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Relevance
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the covalent attachment of ubiquitin to other proteins, and mediates the selective degradation of short-lived and abnormal proteins. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 10% Glycerol, PBS, 1mM DTT, pH 7.2 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Storage instructions
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Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
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Expression system
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E. coli
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBCH6/UBE2E1 protein (His tag) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to UBCH6/UBE2E1
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