Anti-UBE1C antibody (ab38649)

Overview

• Product nameAnti-UBE1C antibodySee all UBE1C primary antibodies ...
• Description
  Rabbit polyclonal to UBE1C
• SpecificityThis antibody is specific for UBE1C (C term).
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  A KLH conjugated synthetic peptide (10-30 aa in length) at the C terminal of last 50 amino acids of UBE1C (Human)

• Positive controlMouse brain tissue lysate and human hepatocarcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody was eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin

Applications

Target

• FunctionCatalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
• Tissue specificityUbiquitously expressed.
• PathwayProtein modification; protein neddylation.
• Sequence similaritiesBelongs to the ubiquitin-activating E1 family. UBA3 subfamily.

Target information above from: UniProt accession Q8TBC4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9039 Human
  • Entrez Gene: 22200 Mouse
  • Omim: 603172 Human
  • SwissProt: Q8TBC4 Human
  • SwissProt: Q8C878 Mouse
  • Unigene: 154320 Human
  • Unigene: 277626 Mouse

• Alternative names
  DKFZp566J164 antibodyEC 6.3.2. antibodyhUba3 antibody

  MGC22384 antibodyNEDD8 activating enzyme E1 catalytic subunit antibodyNEDD8 activating enzyme E1C antibodyNedd8 activating enzyme hUba3 antibodyNEDD8-activating enzyme E1 catalytic subunit antibodyNEDD8-activating enzyme E1C antibodyUBA3 antibodyUBA3 ubiquitin activating enzyme E1 homolog antibodyUBA3_HUMAN antibodyUbiquitin activating enzyme 3 antibodyUbiquitin activating enzyme E1C antibodyUbiquitin-activating enzyme 3 antibodyUbiquitin-activating enzyme E1C antibodyUbiquitin-like modifier-activating enzyme 3 antibody

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Anti-UBE1C antibody images

• 

  Western blot - UBE1C antibody (ab38649)
  Anti-UBE1C antibody (ab38649) + mouse brain tissue lysate
  
  Predicted band size : 52 kDa
  Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 120 kDa,28 kDa,44 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE1C antibody (ab38649)
  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab38649 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.