Anti-UBE2C antibody (ab12290)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2C antibody (ab12290)

Image courtesy of Human Protein Atlas

ab12290 stainning UBEC2 in the nucleus of surface epithelial cells of human esophagus. Paraffin-embbeded tissue was incubated with ab12290 (1/75 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab12290 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

Western blot - UBE2C antibody (ab12290)

All lanes : Anti-UBE2C antibody (ab12290) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 Human embryonic kidney cell line Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size : 20 kDa
Observed band size : 20 kDa
Additional bands at : 55 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Immunocytochemistry/ Immunofluorescence - UBE2C antibody (ab12290)

ICC/IF image of ab12290 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab12290, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 and MCF7 cells.