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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2C antibody (ab12290)
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Western blot - UBE2C antibody (ab12290)
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Immunocytochemistry/ Immunofluorescence - UBE2C antibody (ab12290)
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Product Name
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UBE2C antibody
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See all UBE2C antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to UBE2C
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Immunogen
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Synthetic peptide derived from within residues 150 to the C-terminus of Human UBE2C.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody recognises a band of the correct size (20 kDa) in Hela, A431, Jurkat, Jurkat nuclear and 293 lysates.
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/100.
WB: 1/500. Predicted molecular weight: 20 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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UBE2C catalyzes the covalent attachment of ubiquitin to other proteins. It is required for the destruction of mitotic cyclins. UBE2C carries out the ubiquitin conjugation second step: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE2C antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE2C antibody images, references, products related to ab12290 and other tools.
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UBE2C antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2C antibody (ab12290)
Image courtesy of Human Protein Atlas
ab12290 stainning UBEC2 in the nucleus of surface epithelial cells of human esophagus. Paraffin-embbeded tissue was incubated with ab12290 (1/75 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab12290 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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Western blot - UBE2C antibody (ab12290)
All lanes : UBE2C antibody (ab12290) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
Additional bands at : 55 kDa,70 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - UBE2C antibody (ab12290)
ICC/IF image of ab12290 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab12290, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 and MCF7 cells.
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References for UBE2C antibody (ab12290)
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This product has been used in:
Lin J et al. Expression and effect of inhibition of the ubiquitin-conjugating enzyme E2C on esophageal adenocarcinoma. Neoplasia 8:1062-71 (2006). PubMed: 17217624
If you publish research using ab12290 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to UBE2C
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UBE2C antibody - more information
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Customer reviews (feedback) regarding UBE2C antibody |
Customer FAQs regarding UBE2C antibody |
Protocols for UBE2C antibody |
Price and availability of products related to UBE2C antibody |
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UBE2C antibody - related products:
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UBE2C antibody - other tools:
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Contact Abcam with a Technical Enquiry about UBE2C antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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