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Overview
Product name
Anti-UBE2C antibody
See all UBE2C products (5) ...
Description
Goat polyclonal to UBE2C
Tested applications
WB, IP, ICCmore details
Cross reactivity
Predicted to work with
Human
Immunogen
Synthetic peptide: QETYSKQVTSQEP, corresponding to C terminal amino acids 167-179 of Human UBE2C.
C-QETYSKQV TSQEP
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2.5 µg/mlDetects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).
IP: Use at an assay dependent dilution. ((See Abreview))
ICC: 1/200((See Abreview))
Target
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational
modifications
Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
Target information above from: UniProt accessionO00762
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cyclin selective ubiquitin carrier protein antibody
- dJ447F3.2 antibody
- Mitotic specific ubiquitin conjugating enzyme antibody
see all
Database links
- Entrez Gene: 11065 Human
- Omim: 605574 Human
- SwissProt: O00762 Human
- Unigene: 93002 Human
Anti-UBE2C antibody images:
Western blot - UBE2C antibody (ab3935)
Predicted band size : 21.5 kDa
ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):
Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).
For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
Immunoprecipitation - UBE2C antibody (ab3935)
ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40
Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
Lane 4 = Noc arrested HeLa whole cell lysate.
For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
Immunocytochemistry/ Immunofluorescence - UBE2C antibody (ab3935)
ab3935 at a 1/200 dilution staining human UBE2C in paraformaldehyde fixed HeLa cells by Immunocytochemistry. In the upper panel G2 phase cells can be seen (G1 cells stained only faintly). The lower panel shows a mitotic (metaphase) cell. Diffuse cytoplamic staining with some microtubule localisation can be observed in mitotic cells.
References for Anti-UBE2C antibody (ab3935)
ab3935 has not yet been referenced specifically in any publications.
Publishing research using ab3935? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"