Our Abpromise guarantee covers the use of ab3935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).Can be blocked with Human UBE2C peptide (ab22967).
IP Use at an assay dependent dilution. (See Abreview)
ICC 1/200. (See Abreview)


  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cyclin selective ubiquitin carrier protein antibody
    • dJ447F3.2 antibody
    • Mitotic specific ubiquitin conjugating enzyme antibody
    • UB E2C antibody
    • UBCH 10 antibody
    • UbcH10 antibody
    • UBE 2C antibody
    • Ube2c antibody
    • UBE2C_HUMAN antibody
    • Ubiquitin carrier protein C antibody
    • Ubiquitin carrier protein E2 C antibody
    • Ubiquitin carrier protein E2C antibody
    • Ubiquitin conjugating enzyme E2 C antibody
    • Ubiquitin conjugating enzyme E2C antibody
    • Ubiquitin protein ligase C antibody
    • Ubiquitin-conjugating enzyme E2 C antibody
    • Ubiquitin-protein ligase C antibody
    see all


  • Predicted band size : 21.5 kDa

    ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

    Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
    Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
    Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
    Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

    For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
  • ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40µl of ab3935 saturated protein G dynabeads, by Western blot (ECL):

    Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
    Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
    Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
    Lane 4 = Noc arrested HeLa whole cell lysate.

    For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

  • ab3935 at a 1/200 dilution staining human UBE2C in paraformaldehyde fixed HeLa cells by Immunocytochemistry. In the upper panel G2 phase cells can be seen (G1 cells stained only faintly). The lower panel shows a mitotic (metaphase) cell. Diffuse cytoplamic staining with some microtubule localisation can be observed in mitotic cells.


This product has been referenced in:
  • Zhang Y  et al. Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression. Oncol Rep 33:2529-36 (2015). Read more (PubMed: 25760688) »
  • Argenta PA  et al. Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer. Gynecol Oncol N/A:N/A (2013). Read more (PubMed: 23911795) »

See all 2 Publications for this product

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