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Western blot - UBE2C antibody (ab3935)
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Immunoprecipitation - UBE2C antibody (ab3935)
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Immunocytochemistry/ Immunofluorescence - UBE2C antibody (ab3935)
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Product Name
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UBE2C antibody
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See all UBE2C antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to UBE2C
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Immunogen
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Synthetic peptide: QETYSKQVTSQEP, corresponding to C terminal amino acids 167-179 of Human UBE2C.
BLAST 'C-QETYSKQVTSQEP' with  or 
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Tested applications
(see key)
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ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/200.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).
Please also see review dated 25/02/03 and images below
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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UBE2C catalyzes the covalent attachment of ubiquitin to other proteins. It is required for the destruction of mitotic cyclins. UBE2C carries out the ubiquitin conjugation second step: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE2C antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE2C antibody images, references, products related to ab3935 and other tools.
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UBE2C antibody images:
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 Western blot - UBE2C antibody (ab3935)
Predicted band size : 21.5 kDa
ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):
Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).
For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
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Immunoprecipitation - UBE2C antibody (ab3935)
ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40µl of ab3935 saturated protein G dynabeads, by Western blot (ECL):
Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
Lane 4 = Noc arrested HeLa whole cell lysate.
For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
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Immunocytochemistry/ Immunofluorescence - UBE2C antibody (ab3935)
ab3935 at a 1/200 dilution staining human UBE2C in paraformaldehyde fixed HeLa cells by Immunocytochemistry. In the upper panel G2 phase cells can be seen (G1 cells stained only faintly). The lower panel shows a mitotic (metaphase) cell. Diffuse cytoplamic staining with some microtubule localisation can be observed in mitotic cells.
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Search PubMed (MEDLINE) for references to UBE2C
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UBE2C antibody - more information
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Customer reviews (feedback) regarding UBE2C antibody |
Customer FAQs regarding UBE2C antibody |
Protocols for UBE2C antibody |
Price and availability of products related to UBE2C antibody |
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UBE2C antibody - related products:
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UBE2C antibody - other tools:
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Contact Abcam with a Technical Enquiry about UBE2C antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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