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Overview
Product name
Anti-UBE2E3 antibody
See all UBE2E3 products (3) ...
Description
Rabbit polyclonal to UBE2E3
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal region of Human UBE2E3, conjugated to KLH
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
The antibody was purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Western blot - UBE2E3 antibody (ab71639)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 23 kDa.
Target
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.
Tissue specificity
Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner.
Target information above from: UniProt accessionQ969T4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Homologous to yeast UBC4/5 antibody
- UB2E3_HUMAN antibody
- UBC E4 antibody
see all
Database links
- Entrez Gene: 10477 Human
- Omim: 604151 Human
- SwissProt: Q969T4 Human
- Unigene: 470804 Human
- Unigene: 567831 Human
Anti-UBE2E3 antibody images:
Western blot - UBE2E3 antibody (ab71639)
Anti-UBE2E3 antibody (ab71639) at 1/100 dilution + Jurkat cell lysate at 12.5 µg
Predicted band size : 23 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 15 kDa,45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-UBE2E3 antibody (ab71639)
ab71639 has not yet been referenced specifically in any publications.
Publishing research using ab71639? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"