Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
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Dear Dr Keith Yes,I want use in the beginning ab32219 and ab32058 for the ChiP assay in human liver cell line.Based on the results I want use other antibody as well.I am going to order all the antibody now.Thanks. With regards Bala ________________________________________ From: technical@abcam.com [technical@abcam.com] Sent: Thursday, January 12, 2012 3:27 PM To: Balasubramaniyan, Natarajavelalar Subject: Reply from Abcam to your enquiry regarding ab32058, ab32219, ab75854, ab81371 [CCE3421816] [http://www.abcam.com/ps/cms/emails/CRM-Header.jpg] Dear Sir/Madam We have an answer to your inquiry: Thank you very much for your interest in ab32219, ab32058, ab81371, ab75854. To our knowledge, these products has not been tested in ChIP on human. Therefore, I can offer a discount off a future purchase if you buy now, test in ChIP on human and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test any or each of these products in ChIP on human. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering. 2. Purchase the antibody or antibodies either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Help us improve our service. Rate your experience with us today. Your original inquiry to Abcam: Will these work on ChIP as well? Best regards, Keith Keith Beadle Scientific Support Specialist Abcam Inc. www.abcam.com Abcam Customer Services and Scientific Support Team www.abcam.com/technical [CCE3421816] Discover more at abcam.com |
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ANSWER: |
Ab32058 DISCOUNT CODE: Expiration date: Ab32058 DISCOUNT CODE: Expiration date: Ab81371 DISCOUNT CODE: Expiration date: 14 May 2012 Ab75854 DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and testthese productsinChIP. This code will give you 1 free PRIMARY ANTIBODYper codebefore the expiration date. To redeem this offer, please submit an Abreview foreach product in ChIPand include the associatedcode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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Will these work on ChIP as well? |
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ANSWER: |
Thank you very much for your interest in ab32219, ab32058, ab81371, ab75854. To our knowledge, these products has not been tested in ChIP on human. Therefore, I can offer a discount off a future purchase if you buy now, test inChIP on human and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and testany or each of these productsin ChIP on human. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering. 2. Purchasethe antibody or antibodieseither by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854) at 1/10000 dilution
Lane 1 : U937 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size : 18 kDa
Observed band size : 18 kDa
ab75854, at 1/100 dilution, staining UBE2I / UBC9 in human brain, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
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