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Western blot - UBE2I / UBC9 antibody [EP2938Y] (ab75854)
![Western blot - UBE2I / UBC9 antibody [EP2938Y] (ab75854)](/ps/datasheet/images/ab75854_WB_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody [EP2938Y] (ab75854)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody [EP2938Y] (ab75854)](/ps/datasheet/images/ab75854_IHC_1.jpg)
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Product Name
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UBE2I / UBC9 antibody [EP2938Y]
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See all UBE2I / UBC9 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP2938Y] to UBE2I / UBC9
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Immunogen
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A synthetic peptide corresponding to residues near the N-terminus of human UBE2I / UBC9
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/50.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
IP: 1/60.
WB: 1/5000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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U937, HeLa, Jurkat or HUVEC cell lysates. Human brain tissue.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
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Relevance
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP.
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP2938Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE2I / UBC9 antibody [EP2938Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE2I / UBC9 antibody [EP2938Y] images, references, products related to ab75854 and other tools.
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UBE2I / UBC9 antibody [EP2938Y] images:
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 Western blot - UBE2I / UBC9 antibody [EP2938Y] (ab75854)
All lanes : UBE2I / UBC9 antibody [EP2938Y] (ab75854) at 1/10000 dilution
Lane 1 : U937 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size : 18 kDa
Observed band size : 18 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody [EP2938Y] (ab75854)
ab75854, at 1/100 dilution, staining UBE2I / UBC9 in human brain, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
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Search PubMed (MEDLINE) for references to UBE2I / UBC9
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UBE2I / UBC9 antibody [EP2938Y] - more information
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Customer reviews (feedback) regarding UBE2I / UBC9 antibody [EP2938Y] |
Customer FAQs regarding UBE2I / UBC9 antibody [EP2938Y] |
Protocols for UBE2I / UBC9 antibody [EP2938Y] |
Price and availability of products related to UBE2I / UBC9 antibody [EP2938Y] |
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UBE2I / UBC9 antibody [EP2938Y] - related products:
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UBE2I / UBC9 antibody [EP2938Y] - other tools:
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Contact Abcam with a Technical Enquiry about UBE2I / UBC9 antibody [EP2938Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - UBE2I / UBC9 antibody [EP2938Y] (ab75854)](/index.html?datasheet=75854#ab75854_WB_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody [EP2938Y] (ab75854)](/index.html?datasheet=75854#ab75854_IHC_1.jpg)
