![Western blot - UBE2I / UBC9 antibody [EP2938Y] (ab75854) image](#ab75854_WB_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody [EP2938Y] (ab75854) image](#ab75854_IHC_1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-UBE2I / UBC9 antibody [EP2938Y]
See all UBE2I / UBC9 products (6) ...
Description
Rabbit monoclonal [EP2938Y] to UBE2I / UBC9
Tested applications
WB, IP, IHC-P, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues near the N-terminus of human UBE2I / UBC9
Positive control
U937, HeLa, Jurkat or HUVEC cell lysates. Human brain tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP2938Y
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
Flow Cyt: 1/50.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
IP: 1/60.
WB: 1/5000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
Tissue specificity
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
Target information above from: UniProt accessionP63279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C358B7.1 antibody
- Homologous to yeast UBC9 antibody
- p18 antibody
see all
Database links
- Entrez Gene: 7329 Human
- Entrez Gene: 100044900 Mouse
- Entrez Gene: 22196 Mouse
- Entrez Gene: 25573 Rat
- Omim: 601661 Human
- SwissProt: P63279 Human
- SwissProt: P63280 Mouse
- SwissProt: P63281 Rat
see all
Anti-UBE2I / UBC9 antibody [EP2938Y] images:
Western blot - UBE2I / UBC9 antibody [EP2938Y] (ab75854)
![Western blot - UBE2I / UBC9 antibody [EP2938Y] (ab75854)](/ps/datasheet/Images/75/ab75854/ab75854_WB_1.jpg)
All lanes : Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854) at 1/10000 dilution
Lane 1 : U937 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size : 18 kDa
Observed band size : 18 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody [EP2938Y] (ab75854)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody [EP2938Y] (ab75854)](/ps/datasheet/Images/75/ab75854/ab75854_IHC_1.jpg)
ab75854, at 1/100 dilution, staining UBE2I / UBC9 in human brain, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
References for Anti-UBE2I / UBC9 antibody [EP2938Y] (ab75854)
This product has been referenced in:
- Lee YJet al. Elevated Global SUMOylation in Ubc9 Transgenic Mice Protects Their Brains against Focal Cerebral Ischemic Damage. PLoS One 6:e25852 (2011). WB, IHC-Fr; Mouse.Read more (PubMed: 22016779) »
- Kampmann Uet al. GLUT4 and UBC9 Protein Expression Is Reduced in Muscle from Type 2 Diabetic Patients with Severe Insulin Resistance. PLoS One 6:e27854 (2011). WB; Human.Read more (PubMed: 22114711) »
See all 3 publications for this product
Publishing research using ab75854? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"