Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
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ab51167 |
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ab127405 |
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I have a few questions about this product. 1) Can it be used for Immunocytochemistry? 2) Does it cross react with Humans and Rats? 3) How much is it in GBP (£) and how long would the delivery time be for London, UK? Thanking You |
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ANSWER: |
Thank you for your enquiry. All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as we receive additional data. The information you have requested for can be found from the datasheet: 1) "Tested applications" - ELISA, Immunohistochemistry (Paraffin-embedded sections), Western blot. This product has not been tested in ICC and it may or may not work in this application. We do not guarantee that ab21193 will work in untested applications. 2) "Species Reactivity" - Reacts with Human. Not yet tested in other species. Predicted to react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Xenopus laevis (100% identity with immunogen), Chicken (100% identity with immunogen) and Hamster due to sequence homology. This product reacts with Human but has not been tested on Rat. Based on the immunogen sequence, ab21193 is predicted to crossreact with rat but we do not guarantee this. 3) "Availability" - Select United Kingdom and click on "view price for this country". Please don't hesitate to contact us again if you have further questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 18 kDa
ab21193 (0.1ug/ml) western blot detection of UBE21 from Human kidney lysate (35ug protein in RIPA buffer). primary incubation was 1 hour, detection by chemiluminescence.
Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
ab21193 staining UBE2I / UBC9 in mouse oocyte by Immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% Tween and blocked in 0.1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C. A Cy5® conjugated Donkey polyclonal to goat, diluted 1/400 was used as the secondary.
Motomasa Ihara, Org unknown, Country unknown
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