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Western blot - UBE2I / UBC9 antibody (ab21193)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody (ab21193)
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Immunocytochemistry/ Immunofluorescence - UBE2I / UBC9 antibody (ab21193)
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Product Name
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UBE2I / UBC9 antibody
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See all UBE2I / UBC9 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to UBE2I / UBC9
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Immunogen
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Synthetic peptide: SGIALSRLAQERK, corresponding to N terminal amino acids 2-14 of Human Ube2I.
BLAST 'SGIALSRLAQERK' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Chk, Hm, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/64000.
IHC-P: Use at a concentration of 0.3 - 1 µg/ml. In paraffin embedded Human Kidney,shows nuclear staining of some epithelial cells of glomeruli and renal tubules.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Kidney, Human Lung and Human Testis lysates
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
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Relevance
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP.
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE2I / UBC9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE2I / UBC9 antibody images, references, products related to ab21193 and other tools.
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UBE2I / UBC9 antibody images:
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 Western blot - UBE2I / UBC9 antibody (ab21193)
ab21193 (0.1ug/ml) western blot detection of UBE21 from Human kidney lysate (35ug protein in RIPA buffer). primary incubation was 1 hour, detection by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody (ab21193)
Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Immunocytochemistry/ Immunofluorescence - UBE2I / UBC9 antibody (ab21193)
ab21193 staining UBE2I / UBC9 in mouse oocyte by Immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% Tween and blocked in 0.1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C. A Cy5® conjugated Donkey polyclonal to goat, diluted 1/400 was used as the secondary. Motomasa Ihara, Org unknown, Country unknown
See Abreview
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References for UBE2I / UBC9 antibody (ab21193)
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This product has been used in:
Ihara M et al. UBE2I (UBC9), a SUMO-Conjugating Enzyme, Localizes to Nuclear Speckles and Stimulates Transcription in Mouse Oocytes. Biol Reprod : (2008). WB; Mouse. PubMed: 18703419
If you publish research using ab21193 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to UBE2I / UBC9
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