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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-UBE2I / UBC9 antibody
See all UBE2I / UBC9 products (6) ...
Description
Goat polyclonal to UBE2I / UBC9
Tested applications
ICC/IF, IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Hamster, Dog, Xenopus laevis
Immunogen
Synthetic peptide: SGIALSRLAQERK, corresponding to N terminal amino acids 2-14 of Human Ube2I.
SGIALSRLAQ ERK
Positive control
Human Kidney, Human Lung and Human Testis lysates
General notes
Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (See Abreview.)
IHC-P: Use a concentration of 0.3 - 1 µg/ml.(In paraffin embedded human kidney tissue,shows nuclear staining of some epithelial cells of glomeruli and renal tubules.)
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).(Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.)
ELISA
ELISA: 1/64000ELISA: 1/64000
Target
Function
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
Tissue specificity
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
Target information above from: UniProt accessionP63279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- p18 antibody
- SUMO conjugating enzyme UBC9 antibody
- SUMO-1 conjugating enzyme antibody
see all
Database links
- Entrez Gene: 7329 Human
- Entrez Gene: 100044900 Mouse
- Entrez Gene: 22196 Mouse
- Entrez Gene: 25573 Rat
- Entrez Gene: 380450 Xenopus laevis
- Omim: 601661 Human
- SwissProt: P63279 Human
- SwissProt: P63280 Mouse
see all
Anti-UBE2I / UBC9 antibody images:
Western blot - UBE2I / UBC9 antibody (ab21193)
Predicted band size : 18 kDa
ab21193 (0.1ug/ml) western blot detection of UBE21 from Human kidney lysate (35ug protein in RIPA buffer). primary incubation was 1 hour, detection by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2I / UBC9 antibody (ab21193)
Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Immunocytochemistry/ Immunofluorescence - UBE2I / UBC9 antibody (ab21193)
ab21193 staining UBE2I / UBC9 in mouse oocyte by Immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% Tween and blocked in 0.1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C. A Cy5® conjugated Donkey polyclonal to goat, diluted 1/400 was used as the secondary.
Motomasa Ihara, Org unknown, Country unknown
References for Anti-UBE2I / UBC9 antibody (ab21193)
This product has been referenced in:
- Subramaniam Set al. Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9. J Biol Chem 285:20428-32 (2010). WB; Mouse.Read more (PubMed: 20424159) »
- Yu Fet al. Mir-30 reduction maintains self-renewal and inhibits apoptosis in breast tumor-initiating cells. Oncogene 29:4194-204 (2010). WB; Human.Read more (PubMed: 20498642) »
See all 3 publications for this product
Publishing research using ab21193? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"