Anti-UBE2I / UBC9 antibody (ab21193)
Key features and details
- Goat polyclonal to UBE2I / UBC9
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-UBE2I / UBC9 antibody
See all UBE2I / UBC9 primary antibodies -
Description
Goat polyclonal to UBE2I / UBC9 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Chicken, Hamster, Dog, Xenopus laevis -
Immunogen
Synthetic peptide:
SGIALSRLAQERK
, corresponding to N terminal amino acids 2-14 of Human Ube2I. -
General notes
Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).
A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature. |
Target
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Function
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. -
Tissue specificity
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body. - Information by UniProt
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Database links
- Entrez Gene: 374123 Chicken
- Entrez Gene: 491332 Dog
- Entrez Gene: 7329 Human
- Entrez Gene: 25573 Rat
- Entrez Gene: 380450 Xenopus laevis
- Omim: 601661 Human
- SwissProt: P63283 Chicken
- SwissProt: P63279 Human
see all -
Alternative names
- C358B7.1 antibody
- p18 antibody
- SUMO 1 protein ligase antibody
see all
Images
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Anti-UBE2I / UBC9 antibody (ab21193) at 0.3 µg/ml + Rat ovary lysate at 35 µg
Predicted band size: 18 kDa -
Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Anti-UBE2I / UBC9 antibody (ab21193) at 0.1 µg/ml + Human kidney lysate at 35 µg
Predicted band size: 18 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab21193 has been referenced in 18 publications.
- Yatskevich S et al. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Rep 34:108929 (2021). PubMed: 33789095
- Guérillon C et al. Multisite SUMOylation restrains DNA polymerase ? interactions with DNA damage sites. J Biol Chem 295:8350-8362 (2020). PubMed: 32350109
- Johnson LM et al. Short-term heritable variation overwhelms 200 generations of mutational variance for metabolic traits in Caenorhabditis elegans. Evolution 74:2451-2464 (2020). PubMed: 32989734
- Xu J et al. A Provocative Molecular Link between Mammographic Density and BRCA1-loss associated TNBC. Int J Hum Genet Genet Disord 1:1-8 (2019). PubMed: 33860286
- Heras G et al. Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification. J Mol Cell Biol 11:356-370 (2019). PubMed: 29868881