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Western blot - UBE2I / UBC9 antibody (ab33044)
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Western blot - UBE2I / UBC9 antibody (ab33044)
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Immunocytochemistry/ Immunofluorescence - UBE2I / UBC9 antibody (ab33044)
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Product Name
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UBE2I / UBC9 antibody
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See all UBE2I / UBC9 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to UBE2I / UBC9
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human UBE2I / UBC9.
(Peptide available as ab30701.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Chk, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
NIH 3T3 (Mouse embryonic fibroblast cell line)
MEF1 (Mouse embryonic fibroblast cell line)
PC12 (Rat adrenal pheochromocytoma cell line)
This antibody gave a positive signal in the following tissue lysate:
Testis (Mouse) Tissue Lysate - normal tissue
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
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Relevance
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP.
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE2I / UBC9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE2I / UBC9 antibody images, references, products related to ab33044 and other tools.
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UBE2I / UBC9 antibody images:
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 Western blot - UBE2I / UBC9 antibody (ab33044)
All lanes : UBE2I / UBC9 antibody (ab33044) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 18 kDa
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - UBE2I / UBC9 antibody (ab33044)
ICC/IF image of ab33044 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33044, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to UBE2I / UBC9
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UBE2I / UBC9 antibody - more information
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UBE2I / UBC9 antibody - related products:
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