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I asked about Ubc9 protein(ab3803), not Uba2 which is Ab3804. And I already know Uba2 is cross reactive with His antibody as it is in the data sheet. Let me know about Ubc9 after you check your catalog again. Thanks, |
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ANSWER: |
Thank you for getting back to me. According to the details that I have Ubc9 protein (ab3803) is not epitope tagged. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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I purchased Ubc9 Protein (ab3803) and want to do sumoylation assay and in vitro binding assay. My question is Is there any tag such as His or Flag in the Ubc9 protein? It is important as my substrate is His tagged and I will use His antibody for the detectioin. Thanks |
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ANSWER: |
Thank you for your enquiry. The Uba 2 protein ab3803 is not tagged. However, it does contain his-rich regions which might cross-react with antibodies against the 6x-His epitope tag. According to our datasheet during western analysis with anti-6x-His antibodies, Uba 2 at 80 kDa might be shown. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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could you please tell me whether this antibody is expected to react with UBC9 gene from Drosophila and whether it has been tested in ChIP.
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ANSWER: |
Thank you for your enquiry. Ab3803 is Ubc 9 protein, not an antibody, and has only been tested so far in Western blotting and Functional Studies. If you have any more questions, please do contact us again. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Left Lane: Topo I protein (Topo I fragment: ab3828)
Middle Lane: Sumo 1 sumoylated Topo I (Sumo 1: ab3801; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Right Lane: Sumo 3 sumoylated Topo I (Sumo 3: ab3802; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Note: Topo I is S35-Met labeled.
Left: Human topo I (S35Met-labeled) control.
Right: Sumoylated human topo I (S35Met-labeled)
(7.5
Western Blot: Extracts prepared from CEF cells not transfected (-) or transfected (+) with wild-type Src were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4ºC, then were incubated with 1.0
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