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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Overview
Product name
UBE2I / UBC9 protein
See all UBE2I / UBC9 products (5) ...
Expression host
E. coli
Properties
Purification notes
Human Ubc 9 (Ubiquitin-like protein SUMO-1 conjugating enzyme) is produced from an E. coli expression system, prepared from freshly extracted cell lysates, and purified to homogeneity. The final fraction of the Ubc 9 is an affinity column pool.
Biological activity
The final fraction of enzyme contains a single polypeptide band of 18 kDa.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol
Concentration
Concentration information loading...
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration.
FuncS: Use at an assay dependent concentration.
Application notes
This peptide can be used with studies using ab3801,ab3802,ab3804,ab3819 and ab3828.
Protein info
Function
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
Tissue specificity
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
Target information above from: UniProt accessionP63279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
UBE2I / UBC9 protein images:
Sumoylation assay
Left Lane: Topo I protein (Topo I fragment: ab3828)
Middle Lane: Sumo 1 sumoylated Topo I (Sumo 1: ab3801; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Right Lane: Sumo 3 sumoylated Topo I (Sumo 3: ab3802; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Note: Topo I is S35-Met labeled.
Topo I sumoylation
Left: Human topo I (S35Met-labeled) control.
Right: Sumoylated human topo I (S35Met-labeled)
(7.5
Western Blot: Extracts prepared from CEF cells not transfected (-) or transfected (+) with wild-type Src were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 1.0
References for UBE2I / UBC9 protein (ab3803)
ab3803 has not yet been referenced specifically in any publications.
Publishing research using ab3803? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"