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Overview
Product name
Anti-UBE2M antibody
See all UBE2M products (6) ...
Description
Rabbit polyclonal to UBE2M
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the center region of human UBE2M conjugated to KLH
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Western blot - UBE2M antibody (ab71641)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Target
Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
Pathway
Protein modification; protein neddylation.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Domain
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Target information above from: UniProt accessionP61081
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hUbc12 antibody
- NEDD8 carrier protein antibody
- NEDD8 conjugating enzyme Ubc12 antibody
see all
Database links
- Entrez Gene: 9040 Human
- Omim: 603173 Human
- SwissProt: P61081 Human
- Unigene: 406068 Human
Anti-UBE2M antibody images:
Western blot - UBE2M antibody (ab71641)
Anti-UBE2M antibody (ab71641) at 1/100 dilution + Jurkat cell lysate at 12.5 µg
Predicted band size : 21 kDa
Observed band size : 21 kDa
Additional bands at : 45 kDa,70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-UBE2M antibody (ab71641)
ab71641 has not yet been referenced specifically in any publications.
Publishing research using ab71641? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"