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Overview

  • Product nameUBE2M protein (His tag)See all UBE2M proteins and peptides ...
  • Protein descriptionRecombinant full length Human UBE2M (amino acids 1-183) with an N terminal His tag, 207 amino acids with a predicted MWt of 23.5 kDa including the tag (UniProt P61081; NP_003960).
  • Uniprot accessionP61081
  • Molecular weight23.500kDa inclusive of tags
  • Protein length183 amino acids
  • Expression hostE. coli

    • Properties

  • Purity>95% by SDS-PAGE
  • Purification notesab128447 was purified using conventional chromatography.
  • Mass spectrometryMALDI-TOF
  • E.C. Number6.3.2.-
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 1.17% Sodium chloride
  • Concentration information loading...
  • Sequence notesMGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab128447 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    MS MS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab155783.

    • Protein info

    • Alternative names
        hUbc12NEDD8 carrier proteinNEDD8 conjugating enzyme Ubc12
        NEDD8 protein ligaseNEDD8-conjugating enzyme Ubc12UBC-RS2UBC12UBC12_HUMANube2mubiquitin carrier protein MUbiquitin conjugating enzyme E2 MUbiquitin-conjugating enzyme E2 Mubiquitin-protein ligase Myeast UBC12 homolog
      see all
  • FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • PathwayProtein modification; protein neddylation.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • DomainBoth the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.

    • Target information above from: UniProt accession P61081 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    UBE2M protein (His tag) images

    • SDS-PAGE - UBE2M protein (ab128447)
      SDS-PAGE - UBE2M protein (ab128447)
      15% SDS-PAGE analysis of ab128447 (3 µg).

    References for UBE2M protein (His tag) (ab128447)

    ab128447 has not yet been referenced specifically in any publications.

    Publishing research using ab128447 ? Please let us know so that we can cite the reference in this datasheet

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