Anti-UBE2S antibody (ab197945)
Key features and details
- Rabbit polyclonal to UBE2S
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBE2S antibody
See all UBE2S primary antibodies -
Description
Rabbit polyclonal to UBE2S -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human UBE2S aa 1 to the C-terminus. Full length fusion protein. The identity of the protein fusion partner is GST.
Database link: Q16763 -
Positive control
- Human testis tissue lysate, human thyroid cancer tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/40 - 1/200.
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WB |
1/200 - 1/1000. Predicted molecular weight: 24 kDa.
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Notes |
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IHC-P
1/40 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 24 kDa. |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Post-translational
modificationsAutoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome. - Information by UniProt
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Database links
- Entrez Gene: 27338 Human
- Entrez Gene: 77891 Mouse
- Entrez Gene: 292588 Rat
- Omim: 610309 Human
- SwissProt: Q16763 Human
- SwissProt: Q921J4 Mouse
- SwissProt: B5DFI8 Rat
- Unigene: 396393 Human
see all -
Alternative names
- AA409170 antibody
- E2 EPF antibody
- E2-EPF antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab197945 has been referenced in 1 publication.
- Liu Z & Xu L UBE2S promotes the proliferation and survival of human lung adenocarcinoma cells. BMB Rep 51:642-647 (2018). PubMed: 30545437