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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Overview
Product name
Anti-UBE2V1 antibody
See all UBE2V1 products (5) ...
Description
Mouse polyclonal to UBE2V1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length human UBE2V1 (NP_071887.1).
Positive control
Human kidney tissue lysate and UBE2V1 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 11 kDa.
Target
Function
Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
Tissue specificity
Highly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Cellular localization
Nucleus. Excluded from the nucleolus.
Target information above from: UniProt accessionQ13404
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CIR1 antibody
- CROC-1 antibody
- CROC1 antibody
see all
Database links
- Entrez Gene: 387522 Human
- Entrez Gene: 7335 Human
- Omim: 602995 Human
- SwissProt: Q13404 Human
- Unigene: 420529 Human
- Unigene: 727525 Human
Anti-UBE2V1 antibody images:
Western blot - UBE2V1 antibody (ab88680)
Anti-UBE2V1 antibody (ab88680) at 1 µg/ml + Human kidney tissue lysate at 50 µg
Predicted band size : 11 kDa
Observed band size : 16 kDa (why is the actual band size different from the predicted?)
Western blot - UBE2V1 antibody (ab88680)
All lanes : Anti-UBE2V1 antibody (ab88680) at 1 µg/ml
Lane 1 : UBE2V1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 11 kDa
Observed band size : 11 kDa
References for Anti-UBE2V1 antibody (ab88680)
ab88680 has not yet been referenced specifically in any publications.
Publishing research using ab88680? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"