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Immunoprecipitation - UBE3A antibody (ab10487)
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Product Name
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UBE3A antibody
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See all UBE3A antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to UBE3A
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Immunogen
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Immunogen was a synthetic peptide, which represented a portion of human ubiquitin protein ligase E3A encoded within exon 6 (LocusLink ID 7337).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 1 - 4 µg/mg of cell lysate.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Cytoplasmic fraction from HeLa cells.
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Cellular localization
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Nuclear (probable)
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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UBE3A (Ubiquitin protein ligase E3A) is part of the ubiquitin protein degradation system. The gene for UBE3A is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited mutations of this gene causes Angelman Syndrome. This protein interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. UBE3A has also been shown to interact with the progesterone receptor as a coactivator, and with other members of the nuclear hormone receptor superfamily, enhancing the transcriptional activity of these steroid receptors.
Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Antibodies were affinity purified using the peptide immobilized on solid support.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE3A antibody images, references, products related to ab10487 and other tools.
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UBE3A antibody images:
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 Immunoprecipitation - UBE3A antibody (ab10487)
ab10487 immunoprecipitation of UBE3A (E6AP). Samples: Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody: ab10487 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is UBE3A (E6AP).
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Search PubMed (MEDLINE) for references to UBE3A
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UBE3A antibody - more information
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UBE3A antibody - related products:
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