UBE3A antibody (ab3519)

Product Name 

UBE3A antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to UBE3A

Immunogen 

Full length human E6 AP fusion protein (bacterially expressed).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Western Blot: 1/2000.
Immunoprecipitation: assay dependent.
Not tested in other applications.

Optimal dilution / concentration should be determined by the end user.

By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate.
IMPORTANT: E6-AP is a very labile protein.
Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.

Cellular localization 

Nuclear (probable)

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation

Relevance 

UBE3A (Ubiquitin protein ligase E3A) is part of the ubiquitin protein degradation system. The gene for UBE3A is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited mutations of this gene causes Angelman Syndrome. This protein interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. UBE3A has also been shown to interact with the progesterone receptor as a coactivator, and with other members of the nuclear hormone receptor superfamily, enhancing the transcriptional activity of these steroid receptors.
Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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