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Western blot - UBE3A antibody (ab3519)

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Product Name
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UBE3A antibody
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See all UBE3A antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to UBE3A
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Immunogen
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Full length human E6 AP fusion protein (bacterially expressed).
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Reacts with
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Hu
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Tested applications
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Western Blot: 1/2000. Immunoprecipitation: assay dependent. Not tested in other applications.
Optimal dilution / concentration should be determined by the end user.
By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate. IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.
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Cellular localization
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Nuclear (probable)
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors Neuroscience >> Neurology process >> Neurodegenerative disease >> Other Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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UBE3A (Ubiquitin protein ligase E3A) is part of the ubiquitin protein degradation system. The gene for UBE3A is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited mutations of this gene causes Angelman Syndrome. This protein interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. UBE3A has also been shown to interact with the progesterone receptor as a coactivator, and with other members of the nuclear hormone receptor superfamily, enhancing the transcriptional activity of these steroid receptors. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE3A antibody images, references, products related to ab3519 and other tools.
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UBE3A antibody images:
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Western blot - UBE3A antibody (ab3519)
Western blot of E6-AP in HeLa cell extract using ab3519.
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Search PubMed (MEDLINE) for references to UBE3A
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UBE3A antibody - more information
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Customer reviews (feedback) regarding UBE3A antibody |
Customer FAQs regarding UBE3A antibody |
Protocols for UBE3A antibody |
Price and availability of products related to UBE3A antibody |
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UBE3A antibody - related products:
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UBE3A antibody - other tools:
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Contact Abcam with a Technical Enquiry about UBE3A antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"