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Anti-UBE3A antibody (ab3519)

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Overview

Product name

Anti-UBE3A antibody
See all UBE3A products (6) ...

Description

Rabbit polyclonal to UBE3A

Tested applications

IP, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length human E6 AP fusion protein (bacterially expressed).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.05% sodium azide

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Western blot - UBE3A antibody (ab3519)Western blot - UBE3A antibody (ab3519) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Western Blot: 1/2000.
Immunoprecipitation: assay dependent.
Not tested in other applications.

Optimal dilution / concentration should be determined by the end user.

By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate.
IMPORTANT: E6-AP is a very labile protein.
Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.

Target

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo.

Pathway

Protein modification; protein ubiquitination.

Involvement in disease

Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830]; also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove.

Sequence similarities

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ05086 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • E6AP ubiquitin protein ligase antibody
  • NY REN 54 antigen antibody
  • ANCR antibody
  • Angelman syndrome antibody
  • AS antibody
  • CTCL tumor antigen se37 2 antibody
  • E6 AP antibody
  • E6AP antibody
  • E6AP ubiquitin protein ligase antibody
  • E6AP ubiquitin-protein ligase antibody
  • EPVE6AP antibody
  • FLJ26981 antibody
  • HPVE6A antibody
  • Human papilloma virus E6 associated protein Angelman syndrome antibody
  • Human papilloma virus E6 associated protein antibody
  • Human papillomavirus E6-associated protein antibody
  • NY REN 54 antigen antibody
  • Oncogenic protein associated protein E6 AP antibody
  • Oncogenic protein associated protein E6AP antibody
  • Oncogenic protein-associated protein E6-AP antibody
  • Renal carcinoma antigen NY REN 54 antibody
  • Renal carcinoma antigen NY-REN-54 antibody
  • UBE 3A antibody
  • Ube3a antibody
  • UBE3A protein antibody
  • UBE3A_HUMAN antibody
  • Ubiquitin protein ligase E3A antibody
  • Ubiquitin-protein ligase E3A antibody
see all

Anti-UBE3A antibody images:

  Western blot - UBE3A antibody (ab3519)

Western blot - UBE3A antibody (ab3519)

Western blot of E6-AP in HeLa cell extract using ab3519.

References for Anti-UBE3A antibody (ab3519)

ab3519 has not yet been referenced specifically in any publications.

Publishing research using ab3519? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"