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IHC-P - UBE3A antibody (ab58266)
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Western blot - UBE3A antibody (ab58266)
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Product Name
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UBE3A antibody
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See all UBE3A antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to UBE3A
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Immunogen
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Recombinant fragment: ETFQQLITYK VISNEFNSRN LVNDDDAIVA ASKCLKMVYY ANVVGGEVDT NHNEEDDEEP IPESSELTLQ ELLGEERRNK KGPRVDPLET ELGVKTLDCR , corresponding to amino acids 51-151 of Human UBE3A
BLAST 'ETFQQLITYK VISNEFNSRN LVNDDDAIVA ASKCLKMVYY ANVVGGEVDT NHNEEDDEEP IPESSELTLQ ELLGEERRNK KGPRVDPLET ELGVKTLDCR ' with  or 
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Reacts with
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear (probable)
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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UBE3A (Ubiquitin protein ligase E3A) is part of the ubiquitin protein degradation system. The gene for UBE3A is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited mutations of this gene causes Angelman Syndrome. This protein interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. UBE3A has also been shown to interact with the progesterone receptor as a coactivator, and with other members of the nuclear hormone receptor superfamily, enhancing the transcriptional activity of these steroid receptors.
Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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UBE3A antibody images:
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 IHC-P - UBE3A antibody (ab58266)
UBE3A antibody (ab58266) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung.
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Western blot - UBE3A antibody (ab58266)
Predicted band size : 101 kDa
UBE3A antibody (ab58266) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
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Search PubMed (MEDLINE) for references to UBE3A
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UBE3A antibody - more information
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Customer reviews (feedback) regarding UBE3A antibody |
Customer FAQs regarding UBE3A antibody |
Protocols for UBE3A antibody |
Price and availability of products related to UBE3A antibody |
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UBE3A antibody - other tools:
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Contact Abcam with a Technical Enquiry about UBE3A antibody |
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