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Anti-UBE3B antibody (ab74653)

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Overview

Product name

Anti-UBE3B antibody
See all UBE3B products (2) ...

Description

Rabbit polyclonal to UBE3B

Specificity

ab74653 detects endogenous levels of total UBE3B protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide derived from an internal sequence of human UBE3B.

Positive control

Jurkat cell extracts.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab74653 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases

  • Western blot - UBE3B antibody (ab74653)Western blot - UBE3B antibody (ab74653) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab74653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 123 kDa.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

Target

Relevance

UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.

Alternative names

  • MGC131858 antibody
  • MGC78388 antibody
  • AI449831 antibody
  • AU020130 antibody
  • FLJ45294 antibody
  • Ubiquitin protein ligase E3B antibody
see all

Database links

Anti-UBE3B antibody images:

  Western blot - UBE3B antibody (ab74653)

Western blot - UBE3B antibody (ab74653)

All lanes : Anti-UBE3B antibody (ab74653) at 1/500 dilution

Lane 1 : Jurkat cell extracts
Lane 2 : Jurkat cell extracts with immunising peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 123 kDa
Observed band size : 123 kDa
Additional bands at : 30 kDa,48 kDa. We are unsure as to the identity of these extra bands.

References for Anti-UBE3B antibody (ab74653)

ab74653 has not yet been referenced specifically in any publications.

Publishing research using ab74653? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"