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Western blot - UBE3B antibody (ab74653)
(enlarge)
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Product Name
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UBE3B antibody
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See all UBE3B antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to UBE3B
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Immunogen
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Synthetic peptide derived from an internal sequence of human UBE3B.
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Reacts with
(species key)
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Hu
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Specificity
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ab74653 detects endogenous levels of total UBE3B protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Predicted molecular weight: 123 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell extracts.
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Research areas
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Cell Biology >> Other Antibodies >> Oxidative Stress
Chromatin and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
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Relevance
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UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab74653 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE3B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE3B antibody images, references, products related to ab74653 and other tools.
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UBE3B antibody images:
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 Western blot - UBE3B antibody (ab74653)
All lanes : UBE3B antibody (ab74653) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : Jurkat cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 123 kDa
Observed band size : 123 kDa
Additional bands at : 30 kDa,48 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to UBE3B
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UBE3B antibody - more information
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Customer reviews (feedback) regarding UBE3B antibody |
Customer FAQs regarding UBE3B antibody |
Protocols for UBE3B antibody |
Price and availability of products related to UBE3B antibody |
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UBE3B antibody - related products:
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UBE3B antibody - other tools:
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Contact Abcam with a Technical Enquiry about UBE3B antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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