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Read our guarantee »Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Anti-UBE3B antibody
See all UBE3B products (2) ...
Rabbit polyclonal to UBE3B
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Synthetic peptide corresponding to a region within internal amino acids 720 - 769 (VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE) of Human UBE3B (NP_569733)
VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE
293T cell lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Western blot - UBE3B antibody (ab83834)
(enlarge)
Our Abpromise guarantee covers the use of ab83834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 123 kDa.
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
Western blot - UBE3B antibody (ab83834)

Anti-UBE3B antibody (ab83834) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 123 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 6-18%
ab83834 has not yet been referenced specifically in any publications.
Publishing research using ab83834? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
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Anti-UBE3B antibody (ab83834) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 123 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 6-18%
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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