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Western blot - UBE3B antibody (ab83834)
(enlarge)
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Product Name
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UBE3B antibody
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See all UBE3B antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to UBE3B
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Immunogen
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Synthetic peptide corresponding to a region within internal amino acids 720 - 769 (VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE) of Human UBE3B (NP_569733)
BLAST 'VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 123 kDa.
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293T cell lysate
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Research areas
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Cell Biology >> Other Antibodies >> Oxidative Stress
Chromatin and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
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Relevance
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UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this UBE3B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for UBE3B antibody images, references, products related to ab83834 and other tools.
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UBE3B antibody images:
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 Western blot - UBE3B antibody (ab83834)
UBE3B antibody (ab83834) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 123 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 6-18%
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Search PubMed (MEDLINE) for references to UBE3B
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UBE3B antibody - more information
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Customer reviews (feedback) regarding UBE3B antibody |
Customer FAQs regarding UBE3B antibody |
Protocols for UBE3B antibody |
Price and availability of products related to UBE3B antibody |
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UBE3B antibody - related products:
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UBE3B antibody - other tools:
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Contact Abcam with a Technical Enquiry about UBE3B antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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