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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Overview
Product name
Anti-UBE3C antibody
See all UBE3C products (2) ...
Description
Mouse polyclonal to UBE3C
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length UBE3C protein (Human)
Positive control
UBE3C transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
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Western blot - UBE3C antibody (ab68225)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 48 kDa (predicted molecular weight: 124 kDa).
Target
Relevance
UBE3C is highly expressed in skeletal muscle and detected at much lower levels in kidney and pancreas. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and one IQ domain. UBE3C is an E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. A cysteine residue is required for ubiquitin-thioester formation. There are three named isoforms.
Cellular localization
Nucleus
Alternative names
- KIAA0010 antibody
- KIAA10 antibody
- Ubiquitin protein isopeptide ligase (E3) antibody
- Ubiquitin protein ligase E3C antibody
Database links
- Entrez Gene: 9690 Human
- SwissProt: Q15386 Human
- Unigene: 118351 Human
Anti-UBE3C antibody images:
Western blot - UBE3C antibody (ab68225)
All lanes : Anti-UBE3C antibody (ab68225) at 1/500 dilution
Lane 1 : UBE3C transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 124 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
References for Anti-UBE3C antibody (ab68225)
ab68225 has not yet been referenced specifically in any publications.
Publishing research using ab68225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"