Anti-UCH37 antibody (ab176377)
Key features and details
- Rabbit polyclonal to UCH37
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-UCH37 antibody
See all UCH37 primary antibodies -
Description
Rabbit polyclonal to UCH37 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human UCH37 aa 150-200. The exact sequence is proprietary.
Database link: NP_057068.1 -
Positive control
- 293T, Hela, Jurkat, mouse NIH 3T3 and mouse TCMK-1 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Function
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. -
Sequence similarities
Belongs to the peptidase C12 family. -
Cellular localization
Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 424359 Chicken
- Entrez Gene: 457596 Chimpanzee
- Entrez Gene: 282110 Cow
- Entrez Gene: 478958 Dog
- Entrez Gene: 100059547 Horse
- Entrez Gene: 51377 Human
- Entrez Gene: 56207 Mouse
- Entrez Gene: 100174635 Orangutan
see all -
Alternative names
- UCH37 antibody
- AD-019 antibody
- CGI 70 antibody
see all
Images
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All lanes : Anti-UCH37 antibody (ab176377) at 0.1 µg/ml
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK-1 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 30 seconds -
Detection of UCH37 in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176383 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab176377 has been referenced in 1 publication.
- Yan D et al. Regulation of Bax-dependent apoptosis by mitochondrial deubiquitinase USP30. Cell Death Discov 7:211 (2021). PubMed: 34381024