Anti-UCHL4 antibody (ab231352)
Key features and details
- Rabbit polyclonal to UCHL4
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-UCHL4 antibody -
Description
Rabbit polyclonal to UCHL4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cow, Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse UCHL4 aa 1 to the C-terminus. Expressed in E.coli. N-terminal tags.
Database link: P58321 -
Positive control
- IHC-P: Mouse kidney tissue. WB: Mouse lung lysate; Recombinant mouse UCHL4 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
Target
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Function
Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. -
Tissue specificity
Expressed in various tissues at low level. -
Sequence similarities
Belongs to the peptidase C12 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 93841 Mouse
- SwissProt: P58321 Mouse
- Unigene: 390180 Mouse
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Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L4 antibody
- Ubiquitin thioesterase L4 antibody
- UCH-L4 antibody
see all
Images
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Formalin-fixed, paraffin-embedded mouse kidney tissue stained for UCHL4 using ab231352 at 20 µg/mL in immunohistochemical analysis.
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Anti-UCHL4 antibody (ab231352) at 3 µg/ml + Mouse lung lysate
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution -
Anti-UCHL4 antibody (ab231352) at 2 µg/ml + Recombinant mouse UCHL4 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231352 has not yet been referenced specifically in any publications.