Recombinant Anti-UCKL1 antibody [EPR7384] (ab126754)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7384] to UCKL1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-UCKL1 antibody [EPR7384]
See all UCKL1 primary antibodies -
Description
Rabbit monoclonal [EPR7384] to UCKL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human UCKL1 aa 500-600. The exact sequence is proprietary.
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Positive control
- 293T and HeLa cell lysates; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7384 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May contribute to UTP accumulation needed for blast transformation and proliferation. -
Tissue specificity
Ubiquitous. -
Pathway
Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.
Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1. -
Sequence similarities
Belongs to the uridine kinase family. -
Post-translational
modificationsUbiquitinated by RNF19B; which induces proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. EBNA3 induces isoform 1 translocation to the nucleus, whereas it does change isoform 3 location. - Information by UniProt
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Database links
- Entrez Gene: 54963 Human
- Entrez Gene: 68556 Mouse
- Omim: 610866 Human
- SwissProt: Q9NWZ5 Human
- SwissProt: Q91YL3 Mouse
- Unigene: 504998 Human
- Unigene: 232400 Mouse
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Alternative names
- F538 antibody
- UCK 1L antibody
- UCK1 like antibody
see all
Images
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All lanes : Anti-UCKL1 antibody [EPR7384] (ab126754) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa -
ab126754, at 1/100 dilution, staining UCKL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126754 has not yet been referenced specifically in any publications.