Overview

  • Product nameAnti-UCP2 antibodySee all UCP2 primary antibodies ...
  • Description
    Rabbit polyclonal to UCP2
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UCP2.

  • Positive control
    • This antibody gave a positive signal in Human Adipose and Spleen Tissue Lysates and FFPE human lymph node tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab97931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).

Target

Anti-UCP2 antibody images

  • All lanes : Anti-UCP2 antibody (ab97931) at 1 µg/ml

    Lane 1 : Adipose (Human) Total Protein Lysate - adult normal tissue (ab28980)
    Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-UCP2 antibody (ab97931)

This product has been referenced in:
  • Watanabe N  et al. Flavan-3-ol fraction from cocoa powder promotes mitochondrial biogenesis in skeletal muscle in mice. Lipids Health Dis 13:64 (2014). WB ; Mouse . Read more (PubMed: 24708519) »
  • Nagy L  et al. Glycogen phosphorylase inhibitor N-(3,5-dimethyl-Benzoyl)-N'-(ß-D-glucopyranosyl)urea improves glucose tolerance under normoglycemic and diabetic conditions and rearranges hepatic metabolism. PLoS One 8:e69420 (2013). WB ; Mouse . Read more (PubMed: 23936011) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Specification mouse embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2014

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab97931 has never been tested in EM. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as an...

Read More

Thank you for contacting us.

We currently offer one product which has been tested in IHC-P and is predicted to work in primates. Ab97931 (http://www.abcam.com/UCP2-antibody-ab97931.html).

Though predicted to work based upon sequ...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (Human endotherial cells)
Loading amount 15 µg
Specification Human endotherial cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"