Anti-UCP2 antibody (ab97931)

Overview

• Product nameAnti-UCP2 antibodySee all UCP2 primary antibodies ...
• Description
  Rabbit polyclonal to UCP2
• Tested applicationsIHC-P, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UCP2.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in Human Adipose and Spleen Tissue Lysates and FFPE human lymph node tissue sections.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

Applications

Target

• FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
• Tissue specificityWidely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.
• Sequence similaritiesBelongs to the mitochondrial carrier family.
  Contains 3 Solcar repeats.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession P55851 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 403576 Dog
  • Entrez Gene: 7351 Human
  • Entrez Gene: 22228 Mouse
  • Entrez Gene: 54315 Rat
  • Omim: 601693 Human
  • SwissProt: Q9N2J1 Dog
  • SwissProt: P55851 Human
  • SwissProt: P70406 Mouse

  • SwissProt: P56500 Rat
  • Unigene: 80658 Human
  • Unigene: 171378 Mouse
  • Unigene: 13333 Rat

  see all

• Alternative names
  BMIQ4 antibodyMitochondrial uncoupling protein 2 antibodySLC25A8 antibody

  Solute carrier family 25 member 8 antibodyUCP 2 antibodyucp2 antibodyUCP2_HUMAN antibodyUCPH antibodyUncoupling protein 2 antibodyUncoupling protein 2 mitochondrial proton carrier antibody

  see all

Anti-UCP2 antibody images

• 

  Western blot - UCP2 antibody (ab97931)
  All lanes : Anti-UCP2 antibody (ab97931) at 1 µg/ml
  
  Lane 1 : Adipose (Human) Total Protein Lysate - adult normal tissue (ab28980)
  Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 33 kDa
  Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 3 minutes
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP2 antibody (ab97931)
  IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.