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RabMAbs (5323-1)

Anti-UCP3 antibody [EPR6427] (ab124925)

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Overview

  • Product nameAnti-UCP3 antibody [EPR6427]See all UCP3 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR6427] to UCP3
  • Tested applicationsWB, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human UCP3.

  • Positive controlHuman testis and A673 lysates; A673 cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab124925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa).
IP IP: 1/10 - 1/100.
Flow Cyt Flow Cyt: 1/10 - 1/100.
  • Application notesIs unsuitable for ,ICC/IF or IHC-P.

    • Target

    • FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
    • Tissue specificityOnly in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
    • Involvement in diseaseDefects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    • Sequence similaritiesBelongs to the mitochondrial carrier family.
      Contains 3 Solcar repeats.
    • Cellular localizationMitochondrion inner membrane.

      • Target information above from: UniProt accession P55916 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        Mitochondrial uncoupling protein 3 antibodySLC25A9 antibodySolute carrier family 25 member 9 antibody
        UCP 3 antibodyUCP3 antibodyUCP3_HUMAN antibodyUncoupling protein 3 antibodyUncoupling protein 3 mitochondrial proton carrier antibody
      see all

    Anti-UCP3 antibody [EPR6427] images

    • Western blot - Anti-UCP3 antibody [EPR6427] (ab124925)
      Western blot - Anti-UCP3 antibody [EPR6427] (ab124925)
      All lanes : Anti-UCP3 antibody [EPR6427] (ab124925) at 1/1000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
    • Flow Cytometry - Anti-UCP3 antibody [EPR6427] (ab124925)
      Flow Cytometry - Anti-UCP3 antibody [EPR6427] (ab124925)
      ab124925, at 1/10 dilution, staining UCP3 in permeabilized A673 cells by Flow cytometry.(red).
      A rabbit IgG was used as negative control (green).
    • Other-Anti-UCP3 antibody [EPR6427](ab124925)
      Other-Anti-UCP3 antibody [EPR6427](ab124925)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-UCP3 antibody [EPR6427] (ab124925)

    ab124925 has not yet been referenced specifically in any publications.

    Publishing research using ab124925 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab124925 Submit a Question for ab124925

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    I write to inquiry about your Monoclonal anti-UCP3 antibody (Cat #:5323-1) http://www.epitomics.com/products/product_info/10541   I have seen in the datasheet the WB images testing the antibody on human testis. To my understanding, UCP3 is only express...

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    Our lab has begun retesting the antibody on other samples. They did also want me to mention that UCP3 has been observed in other sample types such as heart and testis (article attached). I will email you again once I have the results, and please le...

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    Hi ,
    Thanks for your quick response. I will isolate mitochondria to further increase specificity. The protocol you suggested me is for cell culture, any other protocol for tissues? my major goal is to look at the expression in tissues. I am ordering ...

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    Thank you for your reply.

    For mitochondrial isolation from tissues there is a product in our catalogue with its correspondent catalogue that may be useful:

    http://www.abcam.com/Mitochondria-Isolation-Kit-for-Tissue-ab110168.html
    <...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"