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Our Abpromise guarantee covers the use of ab3477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Mouse UCP3 peptide (ab4994).
By Western blot, this antibody detects an ~30 kDa protein representing UCP 3 from human and mouse skeletal muscle lysate.
The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection.
Western blot detection of recombinant human UCP-3 using ab3477.
This image is courtesy of an Abreview submitted by Dr ted millsab3477 at a 1/1000 dilution in Western blot. The left hand lane shows the blot of a lysate from muscle mitochondria from a UCP3 knockout mouse, the middle lane shows the same preparation from a wild type mouse, whilst the right hand lane shows a lysate prepared from a wild type rat. An HRP conjugated donkey anti-rabbit antibody was used as the secondary.