Overview

  • Product name
  • Description
    Rabbit polyclonal to UCP3
  • Specificity
    Detects UCP 3 from human, dog and mouse tissues. This antibody does not detect UCP 3 from mouse liver or kidney. Some customers have used this antibody successfully in rat however our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse UCP3 aa 295-308.
    Sequence:

    RALMKVQVLRESPF


    (Peptide available as ab4994)

Properties

Applications

Our Abpromise guarantee covers the use of ab3477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Mouse UCP3 peptide (ab4994).

By Western blot, this antibody detects an ~30 kDa protein representing UCP 3 from human and mouse skeletal muscle lysate.

Target

  • Function
    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • Tissue specificity
    Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • Involvement in disease
    Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similarities
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Form
    UCP3 is preferentially expressed in muscle
  • Alternative names
    • Mitochondrial uncoupling protein 3 antibody
    • SLC25A9 antibody
    • Solute carrier family 25 member 9 antibody
    • UCP 3 antibody
    • UCP3 antibody
    • UCP3_HUMAN antibody
    • Uncoupling protein 3 antibody
    • Uncoupling protein 3 mitochondrial proton carrier antibody
    see all

Images

  • All lanes : Anti-UCP3 antibody (ab3477) at 1 µg/ml

    Lane 1 : L6 membrane enriched extracts
    Lane 2 : C2C12 membrane enriched extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

    The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection.

  • All lanes : Anti-UCP3 antibody (ab3477) at 1/200 dilution

    Lane 1 : Human skeletal muscle lysate with NFDM/TBST
    Lane 2 : Mouse skeletal muscle lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.
    Blocking peptides at 5 % per lane.

    Secondary
    Lane 1 : Goat anti-rabbit IgG peroxidase conjugate, specific to the non-reducing form of IgG. at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG. at 1/10000 dilution


    Predicted band size : 34 kDa
    Additional bands at : 100 kDa (possible non-specific binding).

    Exposure time : 3 minutes
  • Anti-UCP3 antibody (ab3477) at 1 µg/ml + Recombinant human UCP3

    Predicted band size : 34 kDa

    Western blot detection of recombinant human UCP-3 using ab3477.



  • Performed under reducing conditions.

    Predicted band size : 34 kDa


    Exposure time : 30 minutes

    This image is courtesy of an Abreview submitted by Dr ted mills

    ab3477 at a 1/1000 dilution in Western blot. The left hand lane shows the blot of a lysate from muscle mitochondria from a UCP3 knockout mouse, the middle lane shows the same preparation from a wild type mouse, whilst the right hand lane shows a lysate prepared from a wild type rat. An HRP conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Koziel A & Jarmuszkiewicz W Hypoxia and aerobic metabolism adaptations of human endothelial cells. Pflugers Arch 469:815-827 (2017). WB . Read more (PubMed: 28176017) »
  • Rines AK  et al. Snf1-related kinase improves cardiac mitochondrial efficiency and decreases mitochondrial uncoupling. Nat Commun 8:14095 (2017). Read more (PubMed: 28117339) »

See all 49 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 08 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 08 2016

Application
Western blot
Sample
Mouse Cell lysate - nuclear (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2016

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 01 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 01 2014

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Sample
Human Cell lysate - other (Heterologously expressed in Lactococcus lactis)
Specification
Heterologously expressed in Lactococcus lactis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2014

Thank you for your reply.

Regarding the UCP3 results, I agree that the lower band would be the UCP# signal but I am not sure what the higher molecular weight band could be. It may just be non-specific binding of the antibody, or it may be pos...

Read More

Thank you for your call today and for letting us know about the trouble with ab3477. I am hopeful that the background bands will be reduced by loading less protein, further diluting the antibody, and switching to a BSA block. However, if the high backg...

Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up