Overview

  • Product nameAnti-UCP3 antibody
    See all UCP3 primary antibodies
  • Description
    Rabbit polyclonal to UCP3
  • SpecificityDetects UCP 3 from human, rat and mouse tissues. This antibody does not detect UCP 3 from mouse liver or kidney.
  • Tested applicationsFlow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse UCP3 aa 295-308.
    Sequence:

    RALMKVQVLRESPF


    (Peptide available as ab4994)

Properties

Applications

Our Abpromise guarantee covers the use of ab3477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/400.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with UCP3 peptide (ab4994). By Western blot, this antibody detects an ~30 kDa protein representing UCP 3 from rat heart homogenate.

Target

  • FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • Tissue specificityOnly in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • Involvement in diseaseDefects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mitochondrial uncoupling protein 3 antibody
    • Mitochondrial uncoupling protein 3 antibody
    • SLC25A9 antibody
    • Solute carrier family 25 member 9 antibody
    • UCP 3 antibody
    • UCP3 antibody
    • UCP3_HUMAN antibody
    • Uncoupling protein 3 antibody
    • Uncoupling protein 3 mitochondrial proton carrier antibody
    see all

Anti-UCP3 antibody images

  • ab3477 staining UCP3 in differentiated cardiac ventricular cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/400) for 12 hours at 4°C. An Allophycocyanin-conjugated goat polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Gating Strategy: Isotype negative control (white).

    See Abreview

  • Anti-UCP3 antibody (ab3477) at 1 µg/ml + Recombinant human UCP3

    Predicted band size : 34 kDa

    Western blot detection of recombinant human UCP-3 using ab3477.



  • Performed under reducing conditions.

    Predicted band size : 34 kDa


    Exposure time : 30 minutes

    This image is courtesy of an Abreview submitted by Dr ted mills

    ab3477 at a 1/1000 dilution in Western blot. The left hand lane shows the blot of a lysate from muscle mitochondria from a UCP3 knockout mouse, the middle lane shows the same preparation from a wild type mouse, whilst the right hand lane shows a lysate prepared from a wild type rat. An HRP conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-UCP3 antibody (ab3477)

This product has been referenced in:
  • Wojewoda M  et al. Running performance at high running velocities is impaired but V'O(2max) and peripheral endothelial function are preserved in IL-6?/? mice. PLoS One 9:e88333 (2014). WB ; Mouse . Read more (PubMed: 24533077) »
  • Watanabe N  et al. Flavan-3-ol fraction from cocoa powder promotes mitochondrial biogenesis in skeletal muscle in mice. Lipids Health Dis 13:64 (2014). WB ; Mouse . Read more (PubMed: 24708519) »

See all 24 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (4-20% tris-glycine)
Sample Human Cell lysate - other (Heterologously expressed in Lactococcus lactis)
Specification Heterologously expressed in Lactococcus lactis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2014

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - eBioscience Permeabilization Buffer
Sample Human Cell (Differentiated Cardiac Ventricular Cells)
Specification Differentiated Cardiac Ventricular Cells
Gating Strategy Isotype negative control (white)
Preparation Cell harvesting/tissue preparation method: Typsin-EDTA Cell Dissociation
Sample buffer: PBS and 10% FBS
Username

Abcam user community

Verified customer

Submitted Feb 20 2014

Thank you for your reply.

Regarding the UCP3 results, I agree that the lower band would be the UCP# signal but I am not sure what the higher molecular weight band could be. It may just be non-specific binding of the antibody, or it may be pos...

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Thank you for your call today and for letting us know about the trouble with ab3477. I am hopeful that the background bands will be reduced by loading less protein, further diluting the antibody, and switching to a BSA block. However, if the high backg...

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Thank you for contacting Abcam. I have talked to the lab about ab3477 and they have informed me that it is not uncommon for UCP3 antibodies to detect several isoforms of UCP3 in mouse tissue; isoforms 1 & 3 are routinely detected by the antibody. It ma...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Heart)
Loading amount 35 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (10% Gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Andrew Murray

Verified customer

Submitted Mar 17 2009

Thank you for contacting Abcam. Regarding your inquiry, the image that appears on the ab3477 datasheet illustrates Western blot detection of recombinant human UCP-3 using UCP3 antibody. It appears that the antibody is only detecting one band, becaus...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (gastrocnemius muscle)
Loading amount 30 µg
Specification gastrocnemius muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
Username

Dr. ted mills

Verified customer

Submitted Sep 15 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SHSY5Y (neuroblastoma cells))
Specification SHSY5Y (neuroblastoma cells)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Johanna EL Andaloussi-Lilja

Verified customer

Submitted Jan 03 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"