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Synthetic peptide corresponding to Mouse UCP3 aa 295-308.
Our Abpromise guarantee covers the use of ab3477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with UCP3 peptide (ab4994). By Western blot, this antibody detects an ~30 kDa protein representing UCP 3 from rat heart homogenate.|
ab3477 staining UCP3 in differentiated cardiac ventricular cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/400) for 12 hours at 4°C. An Allophycocyanin-conjugated goat polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.
Gating Strategy: Isotype negative control (white).
Western blot detection of recombinant human UCP-3 using ab3477.
This image is courtesy of an Abreview submitted by Dr ted millsab3477 at a 1/1000 dilution in Western blot. The left hand lane shows the blot of a lysate from muscle mitochondria from a UCP3 knockout mouse, the middle lane shows the same preparation from a wild type mouse, whilst the right hand lane shows a lysate prepared from a wild type rat. An HRP conjugated donkey anti-rabbit antibody was used as the secondary.
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