Overview

  • Product nameAnti-UCP3 antibodySee all UCP3 primary antibodies ...
  • Description
    Rabbit polyclonal to UCP3
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human UCP3.

    (Peptide available as ab41426.)

  • Positive control
    • This antibody gave a positive result in the following human tissue lysates: Testis - adult normal; Heart - adult normal; Skeletal Muscle - adult normal. This antibody gave a negative result in the following human tissue lysates: Liver - adult normal; Kidney - adult normal.

Properties

Applications

Our Abpromise guarantee covers the use of ab41038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).Can be blocked with Human UCP3 peptide (ab41426).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • Tissue specificityOnly in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • Involvement in diseaseDefects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localizationMitochondrion inner membrane.
  • Target information above from: UniProt accession P55916 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Mitochondrial uncoupling protein 3 antibody
    • SLC25A9 antibody
    • Solute carrier family 25 member 9 antibody
    • UCP 3 antibody
    • UCP3 antibody
    • UCP3_HUMAN antibody
    • Uncoupling protein 3 antibody
    • Uncoupling protein 3 mitochondrial proton carrier antibody
    see all

Anti-UCP3 antibody images

  • All lanes : Anti-UCP3 antibody (ab41038) at 1 µg/ml

    Lane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
    Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
    Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
    Lane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
    Lane 5 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab41038 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41038, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-UCP3 antibody (ab41038)

ab41038 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Loading amount 20 µg
Specification HUVEC
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 07 2012

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