Anti-UGGT antibody [EPR5105] (ab124879)

Overview

• Product nameAnti-UGGT antibody [EPR5105]See all UGGT primary antibodies ...
• Description
  Rabbit monoclonal [EPR5105] to UGGT
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues in Human UGGT (Q9NYU2).

• Positive controlHuman skeletal muscle, BXPC-3, SH-SY5Y, and MCF7 lysates, Human brain tissue
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR5105
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • ER Proteins

Applications

Target

• FunctionRecognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.
• Tissue specificityHigher levels in pancreas, skeletal muscle, kidney, and brain. Low levels in lung and heart.
• PathwayProtein modification; protein glycosylation.
• Sequence similaritiesBelongs to the glycosyltransferase 8 family.
• DomainThe N-terminal non-catalytic domain is assumed to mediate recognition of proteins with partial folding defects.
• Cellular localizationEndoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment.

Target information above from: UniProt accession Q9NYU2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 56886 Human
  • Entrez Gene: 320011 Mouse
  • Entrez Gene: 171129 Rat
  • Omim: 605897 Human
  • SwissProt: Q9NYU2 Human
  • SwissProt: Q6P5E4 Mouse
  • SwissProt: Q9JLA3 Rat
  • Unigene: 34180 Human

  • Unigene: 261022 Mouse
  • Unigene: 162227 Rat

  see all

• Alternative names
  GT antibodyHUGT1 antibodyUDP glucose glycoprotein glucosyltransferase 1 antibody

  UDP Glucose Glycoprotein Glucosyltransferase antibodyUDP--Glc:glycoprotein glucosyltransferase antibodyUDP-glucose ceramide glucosyltransferase-like 1 antibodyUDP-glucose:glycoprotein glucosyltransferase 1 antibodyUGCGL1 antibodyUGGG1 antibodyUGGG1_HUMAN antibodyUGGT antibodyUggt1 antibodyUGT1 antibodyUGTR antibody

  see all

Anti-UGGT antibody [EPR5105] images

• 

  Western blot - Anti-UGGT antibody [EPR5105] (ab124879)
  All lanes : Anti-UGGT antibody [EPR5105] (ab124879) at 1/10000 dilution
  
  Lane 1 : Human skeletal muscle lysate
  Lane 2 : BXPC-3 cell lysate
  Lane 3 : SH-SY5Y cell lysate
  Lane 4 : MCF7 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 177 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGGT antibody [EPR5105] (ab124879)
  Staining of UGGT in Paraffin-embedded Human brain tissue by Immunohistochemistry using ab124879 at a dilution of 1/100.