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MitoSciences (MS1080)

Anti-UGT antibody [7H2AF11] (ab129729)

Overview

  • Product nameAnti-UGT antibody [7H2AF11]
  • Description
    Mouse monoclonal [7H2AF11] to UGT
  • Tested applicationsIP, In-Cell ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Purified Rat liver mitochondria

  • Positive controlHuman liver homogenate lysate; Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab129729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use at an assay dependent concentration.
In-Cell ELISA In-Cell ELISA: Use a concentration of 1 µg/ml.
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.

Target

  • Alternative names
      Bilirubin-specific UDPGT isozyme 1 antibodyHyodeoxycholic acid-specific UDPGT antibodyUDP-glucuronosyltransferase 1-1 antibody
      UDP-glucuronosyltransferase 1-9 antibodyUDP-glucuronosyltransferase 1-A antibodyUDP-glucuronosyltransferase 1A1 antibodyUDP-glucuronosyltransferase 1A9 antibodyUDP-glucuronosyltransferase 2B28 antibodyUDP-glucuronosyltransferase 2B4 antibodyUGT1A1 antibodyUGT1A9 antibodyUGT2B28 antibodyUGT2B4 antibody
    see all

Anti-UGT antibody [7H2AF11] images

  • Immunoprecipitation image of ab129729. UGT antibody immunocaptured 57 kDa UGT from Human liver homogenate (HLH). Gel stained with Coomassie brilliant blue G.

    Lane 1: Ladder
    Lane 2: Human liver homogenate lysate
  • Immunocytochemistry image of ab129729 stained Hela cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab129729 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates to the endoplasmic reticulum.

References for Anti-UGT antibody [7H2AF11] (ab129729)

ab129729 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"