Anti-UHRF1 antibody (ab153972)
Key features and details
- Rabbit polyclonal to UHRF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-UHRF1 antibody
See all UHRF1 primary antibodies -
Description
Rabbit polyclonal to UHRF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 51-516 of Human UHRF1.
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Positive control
- HeLa whole cell lysate; Human BT483 xenograft; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. -
Tissue specificity
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 ubiquitin-like domain.
Contains 1 YDG domain. -
Developmental stage
Expressed in fetal thymus, liver and kidney. -
Domain
The RING finger is required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3. -
Post-translational
modificationsPhosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29128 Human
- Omim: 607990 Human
- SwissProt: Q96T88 Human
- Unigene: 108106 Human
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Alternative names
- Ac2-121 antibody
- AL022808 antibody
- E3 ubiquitin-protein ligase UHRF1 antibody
see all
Images
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Anti-UHRF1 antibody (ab153972) at 1/2000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 89 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling UHRF1 with ab153972 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.
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Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft labeling UHRF1 with ab153972 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab153972 has been referenced in 2 publications.
- Han L et al. Identification of prognostic miRNA-mRNA regulatory network in the progression of HCV-associated cirrhosis to hepatocellular carcinoma. Transl Cancer Res 11:3657-3673 (2022). PubMed: 36388056
- Antonova A et al. Heat-Shock Protein 90 Controls the Expression of Cell-Cycle Genes by Stabilizing Metazoan-Specific Host-Cell Factor HCFC1. Cell Rep 29:1645-1659.e9 (2019). PubMed: 31693902